Establishment of traumatic brain injury model in rats

Abstract

Abstract: AIM: To establish a rat model for controlled cortical impact brain injury.METHODS: Traumatic brain injury(TBI) model in rats was duplicated by controlled cortical impact brain injury method with a Benchmark^TM stereotaxic impactor.Cognitive function was evaluated at 1, 3, 5, 7, 28 day after TBI with Morris water maze. And then morphological changes was carefully inspected in the injured part and adjacent area.RESULTS: Compared with the sham-operated rats, the time for brain injured rats to find hidden platform were significantly prolonged. Neuronal cells were swollen or loss, and glia cells remarkably increased in the injured sites and hippocampus areas.Neuron and its axon degeneration was observed by using curpric-silver straining technique.CONCLUSION: The rat model for controlled cortical impact brain injury is reproducible and suitable for the pharmacodynamic screening of brain protective drugs.

Key words: Taumatic brain injury, Animal model, Rat, Cupric-silver staining

CLC Number:

GU Bing, JIN Jian-bo, MENG Wei, LI Hua-nan. Establishment of traumatic brain injury model in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(12): 1362-1367.

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