Abstract: AIM: To detect the gene types of 4 genes, gsites which are relating to hereditary deafness by the method of gene array. METHODS: The DNA in the blood of 50 volunteers was extracted. The product of PCR was destructured in the mixture of ice and water. Then the product was hybridized on the gene array. At last the gene type was deduced after scanning. Three DNA samples were carried out for repeated test. RESULTS: 5 kinds of heterozygous mutation in gvolunteers by the experiment of gene type detection of 9 sites in 50 volunteers were detected. These 5 heterozygous mutations were: 5 mutations of GJB2(235delC), mutation of GJB2(299delAT), 1 mutation of SLC26A4(2168A>G), 1 mutation of SLC26A4(IVS7-2 A>G),1 mutation of GJB3(538C>T). CONCLUSION: The gene type of 9 sites was rapidly detected by the method of gene array exactly. And the reproducibility is fine. The results indicated the mutation rate was relatively high; attention should be emphasized on the clinic and patients.

Key words: Hereditary deafness, Gene array, Mutation