High glucose-induced apoptosis of H9c2 cells is closely related to GAPDH nuclear translocation

Abstract

Abstract: AIM: To investigate whether high glucose induces GAPDH nuclear translocation and whether the GAPDH nuclear translocation is related to high glucose-induced apoptosis of H9c2 cells. METHODS: H9c2 cells were cultured for 48 h in high glucose condition, and then cells were collected for apoptosis determination. The generation of reactive oxygen species (ROS) was determined. The expression of GAPDH and iNOS was measured by western blotting. The translocation of GAPDH was determined by immunofluorescence. RESULTS: High glucose induced apoptosis of H9c2 cells. High glucose induced oxidative stress in H9c2 cells and translocation of GAPDH to nucleus. Rotenone and iNOS inhibitor 1400W protected H9c2 cells from death by inhibiting GAPDH translocation to nucleus. CONCLUSION: High glucose induces GAPDH translocation from cytoplasm to nucleus. The inhibition of high glucose-induced apoptosis of H9c2 cells is closely related to GAPDH nuclear translocation.

Key words: H9c2 cells, High glucose, Cell apoptosis, GAPDH translocation, Nitric oxide synthase

CLC Number:

R965.2

SUN Chuan-bo,GAO Jing-hong, HU Liang, ZHANG Jing-wen, WEI Li, LI Qing-ping. High glucose-induced apoptosis of H9c2 cells is closely related to GAPDH nuclear translocation[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(4): 374-379.

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