Expressions of nucleotide-binding oligomerization domain 2 and Toll-like receptor 1 in rat asthma model and the regulation of budesonide

Abstract

Abstract: AIM: To investigate the potential roles of pattern recognition receptor in pathogenesis of asthma inflammation, the expressions of nucleotide-binding oligomerization domain 2(NOD2) and Toll-like receptor 1(TLR1) which affected by budesonide were determined in rat asthma model. METHODS: Rat models of asthma were randomly divided into three groups on average, including asthma group, control group and budesonide treated group. The expressions of NOD2 proteins were detected by immunohistochemical method, and levels of TLR1 were also measured by flow cytometry at blood neutrophil. RESULTS: The expressions of NOD2 proteins in asthma group (0.148±0.009,optical density)at lung tissure were significantly lower than those in control group(0.157±0.006, optical density)(P<0.05), but there were nonsignificance difference of NOD2 protein expressions neither between in budesonide treated group(0.149±0.008 optical density) and asthma group, nor between in budesonide treated group and control group(all P>0.05). Dramaticly, levels of TLR1 in asthma group and in budesonide treated group(74.07±6.26 and 78.54±4.65, optical density, respectively) were significantly lower than those in control group(84.37±4.96, optical density)at blood neutrophil either(P<0.01 or P<0.05, respectively).Moreover, there were a strong positive correlation between levels of NOD2 and TLR1 (n=26, r=0.780, P<0.01 ). CONCLUSION: Levels of NOD2 and TLR1 were decreased in asthmatic rats, the pattern recognition receptor may be a potential role in pathogenesis of asthma inflammation. Perhaps there is a mild effect on up-regulating NOD2 and TLR1 levels by budesonide.

Key words: Asthma, Nucleotide-binding oligomerization domain, Toll-like receptor, Pattern recognition receptor, Rat

CLC Number:

R965.2

ZHANG Zhi-gang, YE Bin, TONG Xia-sheng, KANG Xiao-dong, YE Hui, WANG En-zhi, CHEN Hao, CHEN Qi. Expressions of nucleotide-binding oligomerization domain 2 and Toll-like receptor 1 in rat asthma model and the regulation of budesonide[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(6): 637-642.

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