Experimental study of proliferation and C-erbB2 expression of lung cancer cells induced by cell differentiation agent-Ⅱ

Abstract

Abstract: AIM: To investigate the effects of CDA-Ⅱ (Uroacitide) which is a healthy human urine extraction on proliferation and C-erbB2 expression of human lung cancer cell line A549.METHODS: The effect of CDA-Ⅱ on cellular viability was determined by CCK-8 assay.The change of C-erbB2 expression in A549 cells induced by CDA-Ⅱ was detected by RT-PCR and Western blot.RESULTS: After A549 cells were treated with various concentration of CDA-Ⅱ, cell proliferation was significantly inhibited in a dose-dependent manner.The expression of C-erbB2 was downregulated in A549 cells induced by CDA-Ⅱ.CONCLUSION: CDA-Ⅱ can inhibit the growth of A549 cells associated with downregulation of C-erbB2, which indicates that C-erbB2 is involved in CDA-Ⅱ induced proliferation inhibition of A549 cells.

Key words: CDA-Ⅱ, Human lung cancer cell line A549, Proliferation, C-erbB2

CLC Number:

R965.2

WANG Hong-yan, TU Yong-sheng, XIE Xiao-bin. Experimental study of proliferation and C-erbB2 expression of lung cancer cells induced by cell differentiation agent-Ⅱ[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(8): 847-851.

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