Expression and its significance of GABA signal in gestational trophoblastic cell pathological tissues

Abstract

Abstract: AIM: To explore the effects of GABA_R agonist or antagonist on JAR cells invasion by identifying the expression of GABA,π subunit of GABA_RA, B1 subunit of GABA_RB(GABAB1R) in JAR cells and taking Transwell chamber with GABA or receptor agonist or antagonists. METHODS: The protein expression of GABA, GABRP, GABAB1R were measured by immunocytochemistry and western bolt in JAR cells. The mRNA GABRP, GABAB1R was measured by RT-PCR. The effects of GABA and its receptors agonists and antagonists on JAR cells invasion were assay by Transwell chamber. RESULTS: GABA, GABRP, GABAB1R were expressed in JAR cells. GABA_A receptor agonist muscimol significantly promoted the invasion of JAR cells, while GABA_BR agonist baclofen significantly inhibited JAR cell migration. CONCLUSION: GABA signal may be associated with the pathogenesis of trophoblast-associated diseases of pregnancy.

Key words: Trophoblast diseases, γ-amino butyric acid, Invasion

CLC Number:

R965.2

LUO Wen-ping, LIU Zhen-zhen, PENG Hong-ying, TAN Dong-mei, ZHAO Hai, TAN Yi. Expression and its significance of GABA signal in gestational trophoblastic cell pathological tissues[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(3): 274-279.

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