Experimental study on the effects of amiodarone on thyroid function and morphology in SD rats

Abstract

Abstract: AIM: To investigate the effects of amiodarone on thyroid hormone secretion, signaling protein expression and morphology.METHODS: SD rats were divided into low-dose group (50 mg·kg^-1·d^-1) and high-dose group (200 mg·kg^-1·d^-1), feeding amiodarone for 14 weeks respectively. Free triiodothyronine (FT3), free thyroxine (FT4), total protein (TP), glycerin three greases (TG) were measured from SD rats' blood. Analysis of the expression for Bcl-2 protein and the activity of Caspase-3 protein were observed from SD rats' thyroid gland, and pathology examination were performed.RESULTS:After amiodarone medication, SD rats' appearance were hair removal, sluggish, weight loss, serum total protein decreased, FT3 decreased with amiodarone's dose increasing, FT4 in the low-dose group slightly increased, while in the high-dose group decreased. The positive expression of Bcl-2 in low-dose group and high-dose group, while negative expression of Caspase-3 in all of them(P>0.05).CONCLUSION: Effects of amiodarone on SD rats' thyroid were manifested as thyroid hormone metabolism were inhibited with the dose increasing, and the expression of Bcl-2 protein were enhanced, while the apoptosis by Caspase-3 induced had not been initial.

Key words: Amiodarone, Thyroid, Bcl-2, Caspase-3

CLC Number:

R965.2

JIANG Li-qin, WANG Zhi-gang, JI Yu-zhen. Experimental study on the effects of amiodarone on thyroid function and morphology in SD rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(5): 508-512.

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