Abstract: AIM: To observe the effect of Yang Ren Tiao Chong Decoction on climacteric syndrome combined with yin deficiency and internal heat in rats.METHODS: 120 female SD rats were randomly divided into six groups: control group, model group, Yang Ren Tiao Chong Decoction low-dose group (0.81 g/kg), mid-dose group (1.62 g/kg), high-dose group (3.24 g/kg) and Kun Bao Pill group as positive control (0.16 g/kg). From the 14th day after castration surgery, the rats from drug group were perfused 0.5 mL/100 g corresponding drugs respectively once daily for 30 days. The water intake, rectal temperature, rate of keratinocytes vaginal epithelial cells and uterine coefficient were all detected, and a variety of serum hormone levels were measured by radioimmunoassay and ELISA.RESULTS: After Yang Ren Tiao Chong Decoction treatment, the water intake of model rats was reduced (P<0.05) and the rectal temperature was declined (P<0.05). With high-dose Yang Ren Tiao Chong Decoction, the uterine coefficient was increased by 68.10% [ (1.95±0.21) mg/g vs (1.16±0.11) mg/g, P<0.01] and the rate of keratinocytes vaginal epithelial cells was increased by 48.66%, too[(44.94±5.26)% vs (30.23±3.86)%, P<0.01]. In addition, the level of E₂ was increased by 43.63% [(60.24±7.36)pmol/L vs(41.94±5.82)pmol/L, P<0.05], but the serum levels of FSH, LH and PRL were all decreased, and there were no significant change in the levels of leptin and insulin (P>0.05).CONCLUSION: Yang Ren Tiao Chong Decoction has a better intervention on neuroendocrine disorders of rats with climacteric syndrome combined with yin deficiency and internal heat.

Key words: Yang Ren Tiao Chong Decoction, Yin deficiency and internal heat, Climacteric syndrome, Hormone