Roles of peripheral Th17/Treg in patients with stable chronic obstructive pulmonary disease

Abstract

Abstract: AIM: To observe the level of Th17 and Treg cells in peripheral blood of stable chronic obstructive plumonary disease(COPD) patients. METHODS: Froty patients that were diagnosed as COPD were selected as expperiment group from January 2010 to December 2012, another 40 healthy people were selected as control group at the same period. Peripheral blood was collected from two groups to analyze Th17 and the proportion of Treg cell subset by flow cytometry, to detect serum IL-17, TGF-β by ELISA, and detected the transcription factor Foxp3 and RORγT mRNA by RT-PCR. RESULTS: The percentage of Th17 in peripheral blood of SCOPD patients was (3.14±0.31)%, significantly higher than (1.23±0.24)% in control group, the difference was statistically significant (P<0.05); proportion of Treg in peripheral blood of SCOPD patients was (6.27±0.32)%, significantly higher than that in the control group (3.43±0.32) %, the difference was statistically significant (P<0.01); SCOPD patients with levels of IL-17 (89.36±4.13) pg/mL, significantly higher than the control group (30.35±2.53) pg/mL, the difference was statistically significant (P<0.05); SCOPD patients with the TGF-β levels (681.45±41.65) pg/mL, significantly higher than the population of control group (519.36±30.43) pg/mL, the difference there was statistically significant (P<0.01); SCOPD patients with transcription factor Foxp3 and RORγT mRNA levels were significantly higher the control group, the difference was statistically significant (all P<0.01). CONCLUSION: Th17, Treg cells and IL-17, TGF-β levels in SCOPD patients increased and the transcription factor Foxp3 and RORγT mRNA expression levels also increased. They played an important role in clinical research.

Key words: COPD, Th17, Treg, IL-17, TGF-β

CLC Number:

R563.5

CHEN Xue-song, WU Ning, QI Yu-qin, ZHANG Xing-hu, WAN Wen-hui, QIAN Xiao-ming. Roles of peripheral Th17/Treg in patients with stable chronic obstructive pulmonary disease[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(12): 1383-1388.

References

[1] Jarad N. Chronic obstructive pulmonary disease (COPD) and old age [J]. Chron Respir Dis, 2011, 8(2): 143-151.
[2] Liu LL, Qin Y, Cai JF, et al. Th17/Treg imbalance in adult patients with minimal change nephrotic syndrome[J]. Clin Immunol, 2011, 139(3): 314-320.
[3] Ratajczak P, Janin A, Peffault de Latour R, et al. Th17/Treg ratio in human graft-versus-host disease[J]. Blood, 2010, 116(7): 1165-1171.
[4] Li J, Lai X, Liao W, et al. The dynamic changes of Th17/Treg cytokines in rat liver transplant rejection and tolerance[J]. Int Immunopharmacol, 2011, 11(8): 962-967.
[5] 中华医学会呼吸病学分会慢性阻塞性肺疾病组. 慢性阻塞性肺疾病诊治指南(修订版)[J]. 中华内科杂志, 2007, 46(3): 254-261.
[6] Li N, Bian H, Zhang J, et al The Th17/Treg imbalance exists in patients with heart failure with normal ejection fraction and heart failure with reduced ejection fraction[J]. Clin Chim Acta, 2010, 411(23/24): 1963-1968.
[7] Li Q, Wang Y, Chen K, et al. The role of oxidized low-density lipoprotein in breaking peripheral Th17/Treg balance in patients with acute coronary syndrome[J]. Biochem Biophys Res Commun, 2010, 394(3): 836-842.
[8] Zhang F, Meng G, Strober W. Interactions among the transcription factors Runx1, RORgammat and Foxp3 regulate the differentiation of interleukin 17-producing T cells[J]. Nat Immunol, 2008, 9(11): 1297-1306.
[9] Cosio MG, Saetta M, Agusti A. Immunologic aspects of chronic obstructive pulmonary disease[J]. N Engl J Med, 2009, 360(23): 2445-2454.
[10] Chatila TA. Role of regulatory T cells in human diseases[J]. J Allergy Clin Immunol, 2005, 116(5): 949-959.
[11] Vargas-Rojas MI, Ramirez-Venegas A, Limon-Camacho L, et al Increase of Th17 cells in peripheral blood of patients with chronic obstructive pulmonary disease[J]. Respiratory Medicine, 2011, 105(11): 1648-1654.
[12] Barczyk A, Pierzchala W, Sozanska E. Interleukin-17 in sputum correlates with airway hyper responsiveness to methacholine [J]. Respir Med, 2003, 97(9): 726-733.
[13] 石建邦,徐晓玲,夏淮玲,等.慢性阻塞性肺疾病稳定期患者和肺功能正常吸烟者外周血中Th17细胞的表达及意义[J].安徽医科大学学报,2012,47(7):835-838.
[14] 陈钢,谢灿茂,罗志扬,等. 慢性阻塞性肺疾病加重期和稳定期Th17 和Treg 细胞的表达[J].中山大学学报(医学科学版),2013,34(6):917-921.
[15] Miossec P.Interleukin-17 in rheumatoid arthritis: If T cells were to contribute to inflammation and destruction through synergy[J].Arthritis Rheum,2003,48(3) : 594-601.
[16] Domagala-Kulawik J, Hoser G, Dabrowska M, et al. CD₄⁺/CD₂₅⁺ cells in systemic inflammation in COPD[J]. Scand J Immunol, 2011, 73(1): 59-65.