The role of ginkgo biloba extract on the hepatic GRP78 and CHOP expression in rats with liver fibrosis

Abstract

Abstract: AIM: To observe the effect of ginkgo biloba extract (GbE) on expression of GRP78 and CHOP in endoplasmic reticulum (ER) stress-related pathway in rats with liver fibrosis, and to explore the mechanism of anti-hepatic fibrosis of GbE. METHODS: The model with liver fibrosis in rats were induced by intraperitoneal injection of 40% CCl₄。Meanwhile, all the rats were administrated with different doses of GbE (200, 100 and 50 mg·kg^-1·d^-1 body weight) by gavage. After the experiment, the mRNA and protein expressions of hepatic GRP78 and CHOP were detected with RT-PCR and immunohistochemical method. RESULTS: The mRNA and protein expression of hepatic GRP78 and CHOP in the rats of the model were more obviously enhanced than in the normal (P<0.05, P<0.01), which were obviously decreased after the treatment with GbE (P<0.05, P<0.01). CONCLUSION: ERS reaction occurs on the liver of rats with liver fibrosis. And that GbE down-regulate the mRNA and protein expressions of hepatic GRP78 and CHOP probably is one of its mechanism of anti-liver fibrosis.

Key words: liver fibrosis, endoplasmic reticulum stress, GRP78, CHOP, GbE

CLC Number:

R965.2

YE Lei, LI Jian-shuang, CHEN Zhi-yun, YAN Mao-xiang, HE Pei-hui. The role of ginkgo biloba extract on the hepatic GRP78 and CHOP expression in rats with liver fibrosis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(4): 377-380.

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