Effects of exogenous H2S donor NaHS on learning and memory in rats after cardiopulmonary resuscitation

Abstract

Abstract: AIM: To investigate the effects of exogenous hydrogen sulfide on learning and memory in rats after cardiopulmonary resuscitation(CPR) and explore its mechanism. METHODS: Experimental rats were randomly divided into five groups: control group, model group(CPR), NaHS low dose group,middle dose group and high dose group . The ability of learning and memory was tested by Morris water maze; The activity of super oxide dismutase (SOD) and the content of malondialdehyde (MDA) were measured with colorimetry; the morphological alterations in hippocampus slices were assessed by microscope. RESULTS:Compared with control group, the escape latency in model group was longer, the residence time in original platform quadrant and the times of passing the position of platform were less, SOD activity was decreased and the content of MDA was increased in model group. Compared with model group, the escape latency in treatment groups was shorter, the number of crossings over the target area and the residence time in original platform quadrant were increased. SOD activity and the content of MDA was increased and decreased, respectively. Morphological changes were significantly attenuated by NaHS in treatment groups.CONCLUSION: CPR can impair the ability of learning and memory of rats, and exogenous H₂S donor NaHS can reduce the impairment of learning and memory of rats after CPR. The mechanism may be related to that H₂S has the effects of anti-lipid peroxidation and scavenging free radical.

Key words: cardiopulmonary resuscitation, hydrogen sulfide, morris water maze, learning and memory

CLC Number:

R965.2

WU Chang-jiang, HUANG Chao-fa, ZHU Tuan-jie, WANG Hui. Effects of exogenous H₂S donor NaHS on learning and memory in rats after cardiopulmonary resuscitation[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(7): 727-732.

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Effects of exogenous H₂S donor NaHS on learning and memory in rats after cardiopulmonary resuscitation

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