Using patch clamp technique to investigate influence of helicid to electrophysiological function of hERG potassium channel

Abstract

Abstract: AIM: To investigate the impact of natural medicine helicid on hERG potassium currents and potential cardiac toxicity.METHODS: hERG gene was stably transfected in CHO cells. The whole-cell patch-clamp technique was applied to detect effects on hERG channel current of different concentrations helicid.RESULTS: IC₅₀ of helicid on hERG channels was 47 μmol/L, which was more than 1000 times higher than the peak concentration in human body at clinical dose.CONCLUSION: It is less likely to encounter cardiac safety issues via the hERG channel inhibition of helicid in clinical application.

Key words: helicid, hERG potassium channels, patch-clamp technique, cardiotoxicity

CLC Number:

R965.2

JIA Yuan-wei, WANG Min-hui, XIE Hai-tang, SHEN Jie, ZHANG Xiao-nan, SI Jin-hui, ZHANG Cui-feng. Using patch clamp technique to investigate influence of helicid to electrophysiological function of hERG potassium channel[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(3): 252-255.

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