Abstract:

AIM: To investigate the expression and significance of transient receptor potential channel protein V1 (TRPV1) and transient receptor potential channel protein A1 (TRPA1) in dorsal root ganglion (DRG) neurons in rats with endometriosis. METHODS: Female, mature unmated Sprague-Dawley healthy 40 rats were divided into model group and sham operation group. Model group underwent autologous transplantation methods to establish the rat model of endometriosis, while the sham operation group underwent open surgery. The expressions of DRG TRPV1 and TRPA1 were detected. And the hot plate pain threshold and tail flick latency change of rats were observed. RESULTS:The positive expression rates of TRPV1 and TRPA1 in the model group were significantly higher than those in the sham operation group (P<0.05). Before the operation, hot plate pain threshold and tail flick latency of two groups were similar (P>0.05); after the operation, the hot plate pain threshold and tail flick latency of rats in the model group decreased significantly (P<0.05), and were significantly less than those in the sham operation group (P<0.05). Before the TRPV1, TRPA1 antagonists treatment, the hot plate pain threshdd and tail flick latency were similar in the two groups (P>0.05), and after the antagonists treatment, the results were the same. In the model group, before and after the use of TRPV1, TRPA1 antagonist, hot plate pain threshold, tail flick latency had no significant change (P>0.05). CONCLUSION: TRPV1 and TRPA1 are highly expressed in the rat model of endometriosis, and the expression of TRPV1 and TRPA1 in the rat model of endometriosis can be decreased by using TRPV1 and TRPA1 antagonist, and the sensitivity to pain is decreased.

Key words: endometriosis, animal model, dorsal root ganglion neurons, transient receptor potential channel protein V1, transient receptor potential channel protein A1