Effect of Fuyuan Huoxue decoction on expression of p-Src and p-Erk in injured sciatic nerve in rats

Abstract

Abstract:

AIM: To study the effect of Fuyuan Huoxue decoction on sciatic nerve regeneration in sciatic nerve injury rat model and its molecular mechanisms. METHODS: The right sciatic nerves of sixty Sprague Dawley rats were performed with transection anastomosis, and the rats were randomly divided into three groups: sciatic nerve transection anastomosis model group (Model), Fuyuan Huoxue decoction treatment group (FHD) and mecobalamine treatment group (MB), with twenty rats in each group postoperatively. The rats of the FHD group were fed with FHD (ig, 9 g·kg-1·d-1) for two to eight weeks, the rats of the MB group were fed with mecobalamine (ig, 6.25×10-4 g·kg-1·d-1) and the rats of the model group were fed with equivalent solvent (ig) daily. The right sciatic nerves of another twenty rats in the sham group were exposed without any treatment and then the wounds were closed. The serum IL-1β and TNF-α level of the rats were detected with ELISA method after three days of administration; the sciatic nerves functional index was investigated after two, four and eight weeks of the treatment, respectively; the mRNA expression of IL-1β and TNF-α and the proteins expression of the p-Src, p-Erk1/2 of the nervous tissue 2 cm up and down anastomotic stoma were analyzed with RT-PCR and Western blot method; the pathological changes of the sciatic nerve and its innervated muscle were observed after eight weeks of the treatment. RESULTS:FHD and mecobalamine could significantly improve the sciatic nerves functional index, facilitated the nerve fibers growth, increased the diameter and cross-sectional area of the gastrocnemius muscle fiber markedly (P<0.01); FHD decreased the serum IL-1β and TNF-α level, lowed the IL-1β and TNF-α mRNA expression (P<0.01), the p-Src and p-Erk1/2 expression (P<0.01) of the nervous tissue 2 cm up and down anastomotic stoma. CONCLUSION: FHD can promote the sciatic nerve regeneration after the transection anastomosis, and the mechanism might be related to reducing inflammatory reaction, and inhibition of the Src and Erk1/2 phosphorylation in the rat sciatic nerve anastomosis.

Key words: Fuyuan Huoxue decoction, sciatic nerve transection anastomosis, inflammatory reaction, phosphorylated Src, phosphorylated Erk

CLC Number:

R965.2

ZHONG Shuzhi, PEI Dong, CHAO Gao, ZHOU Jing, HONG Zongyuan. Effect of Fuyuan Huoxue decoction on expression of p-Src and p-Erk in injured sciatic nerve in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(7): 731-737.

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