Effects of pinocembrin on mRNA expression of genes related to endoplasmic reticulum stress-mediated apoptosis in acute injury induced by focal cerebral ischemia-reperfusion

doi:10.12092/j.issn.1009-2501.2018.01.007

Abstract

Abstract:

AIM: To investigate the effects of pinocembrin on mRNA expression of several genes related to endoplasmic reticulum stress-mediated apoptosis in acute injury induced by focal cerebral ischemia-reperfusion. METHODS: Fifty male Sprague-Dawley rats, weighing 260-280 g were divided into five groups: sham operation group; I/R group; I/R + pinocembrin (1, 3 and 10 mg/kg) group. Focal cerebral I/R model was established by occlusion of the middle cerebral artery(MCAO) for 2 h followed by 6 h reperfusion. Pinocembrin was administered intravenously at different doses at the onset of reperfusion. The mRNA expression of gene GRP78, CHOP, ATF4, XBP-1 was then detected by RT-PCR method in penumbra cortex, respectively. RESULTS: Pinocembrin significantly modulated the levels of mRNA expression of these genes by increasing GRP78 and attenuating CHOP expression along with ATF4, and XBP-1 (P<0.05 or P<0.01). CONCLUSION: Pinocembrin can protect against I/R injury, and the possible mechanism might be attributed to its regulation of mRNA expression of genes related to endoplasmic reticulum stress-mediated apoptosis in penumbra area.

Key words: pinocembrin, endoplasmic reticulum stress, focal cerebral ischemia reperfusion, GRP78, CHOP, ATF4, XBP-1

CLC Number:

R965.2

WU Caixia, DU Guanhua. Effects of pinocembrin on mRNA expression of genes related to endoplasmic reticulum stress-mediated apoptosis in acute injury induced by focal cerebral ischemia-reperfusion[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(1): 34-40.

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