Study on interleukin-17A during early inflammatory response in mice with LPS-induced acute lung injury

doi:10.12092/j.issn.1009-2501.2018.02.006

Abstract

Abstract:

AIM: To observe the dynamic changes of IL-17A in lipopolysaccharide (LPS)-induced acute lung injury(ALI) in mice, and to assess the correlation of IL-17A during early inflammatory response of ALI. METHODS: Mice were used to establish the ALI group and its control group by intratracheal administration of LPS and normal saline, respectively. The level of IL-17A in bronchoalveolar lavage fluid (BALF) and MCP-1 in serum, histopathological changes in lung tissue, lung wet weight /dry weight ratio (W/D), the expression of intercellular adhesion molecule 1 (ICAM-1) and the myeloperoxidase (MPO) activity in lung homogenates would be tested after modeling for 3, 8, 14, 24 h. RESULTS: Compared with the controls, the level of IL-17A in BALF increased (P<0.05) and the level of MCP-1 in serum increased significantly (P<0.01), and as time went by, the three above decreased gradually, and the histological examination of lung showed inflammatory changes, and W/D ratio, expression of ICAM-1, MPO activity aggravated gradually in the ALI group (P<0.05). CONCLUSION: The level of IL-17A in BALF increased significantly during early ALI, and this shows that IL-17A may play an important role in the progress of early inflammatory response in LPS-induced ALI.

Key words: acute lung injury, lipopolysaccharide, IL-17A

CLC Number:

R965.2

ZHENG Shuang, FU Qinlei, ZHOU Lingping, PENG Chuanpeng, XU Honglei. Study on interleukin-17A during early inflammatory response in mice with LPS-induced acute lung injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(2): 148-153.

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