Study on differential expression of the mi-RNA in the deep second degree burns

doi:10.12092/j.issn.1009-2501.2018.12.001

Abstract

Abstract:

AIM:To study the differential expression profile of the miRNA in deep second-degree burns and normal skin tissue, and to investigate the effects of differential expression of the miRNA on wound healing. METHODS: The differential expression of miRNA in deep second-degree burns and normal skin tissue were screened by gene chip detection technology, and the expression of some miRNA was verified by RT-PCR. Target gene prediction was carried out through gene databases TargetScan, miRanda and mirbase. The targeting function analysis and signaling pathway analysis for the predicted target genes were completed with the help of GO-analysis.RESULTS:Compared with the normal group, there were 41 miRNAs genes up-regulated and 124 miRNAs genes down-regulated in the deep second-degree group, and greater differences were shown in some genes, including miR-210-3P, miR-99a-5P, miR-99b-3P, miR-99b-5P, miR-143-5P, miR-29c-5P, miR-15b-5P, miR-323a-3P, miR-424-5P, miR-125b-1-3P, miR-125a-3P, miRNA-184, which were involved in the inflammatory response, immune response and the healing process. CONCLUSION:The regulatory mechanism of miRNA with differential expression and its corresponding target genes in the healing of burn wounds was confirmed at the gene level, which provided targets for the precise treatment for burn wounds.

Key words: the deep second-degree burns, skin, miRNA

CLC Number:

R965.2
R96

PAN Xiaoqiu, ZHAI Hongjun, LI Lihui, BAN Yaolin. Study on differential expression of the mi-RNA in the deep second degree burns[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(12): 1321-1328.

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