Effects of NBP on energy metabolism of human brain microvascular endothelial cells under the condition of glucose deprivation

doi:10.12092/j.issn.1009-2501.2018.12.002

Abstract

Abstract:

AIM: To study the effect and protective effect of butylphthalide (NBP) on the energy metabolism of human cerebral microvascular endothelial cells (HBMEC) under the condition of glucose deprivation (OGD). METHODS: HBMEC cells were treated by OGD. The cells were divided into control group, model group, low dose group, middle dose group and high dose group. The control group was normal cultured, the model group were OGD treated, the low dose group received OGD+5 mol/L NBP, the middle dose group received OGD+10 mol/L NBP, and the high dose group received OGD+20 mol/L NBP. The CCK-8 kit was used to detect the cell viability.The leaking rate of lactate dehydrogenase (LDH), sodium potassium ATPase (Na+-K+-ATPase), calcium triphosphatase (Ca2+-ATPase) activity and intracellular calcium concentration were detected acording to the description of kit,respectively. The flow cytometry was used to detect the apoptosis rate and Hoechst33342 staining. The high performance liquid chromatography was used to detect the contents of ATP, ADP and AMP in the cells, and the EC value was measured.RESULTS:The cell activity, Na+-K+-ATPase and Ca2+-ATPase in the model group was decreased, but the intracellular calcium concentration, the rate of apoptosis and the leakage rate of LDH were significantly increased. After NBP intervention, the activity of cell, the activity of Na+-K+-ATPase and Ca2+-ATPase, the concentration of calcium ion in the cell and the rate of cell apoptosis were reduced. The content of ATP, ADP and EC value in the cells was increased while the content of AMP was decreased in a dose-dependent manner. CONCLUSION: NBP can protect HBMEC injury under OGD, and its mechanism is related to improving energy metabolism and inhibiting intracellular calcium.

Key words: butylphthalide, glucose and oxygen deprivation, human brain microvascular endothelial cells, energy metabolism

CLC Number:

R965.2

HAN Chenyang, ZHANG Xiaoling, GUAN Qiaobin, WANG Yanping. Effects of NBP on energy metabolism of human brain microvascular endothelial cells under the condition of glucose deprivation[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(12): 1329-1334.

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