Establishment of in situ perfused rat intestine-liver model for evaluation on chlorogenic acid metabolism

Abstract

Abstract: AIM: To establish a model of in situ perfused rat intestine-liver preparation and HPLC-MS method in the determination of chlorogenic acid, caffeic acid,hippuric acid and ferulic acid in perfusate, and investigate chlorogenic acid metabolism in intestine and liver of rats by the model.METHODS: Chlorogenic acid and its metabolites (caffeic acid, ferulic acid and hippuric acid)were detected by HPLC-MS from samples of in situ perfused rat intestine-liver model.RESULTS: Main active metabolite of chlorogenic acid is ferulic acid in in situ perfused rat intestine-liver model after intraduodenal injection to rats, and trace caffeic acid and abundant hippuric acid were also found in perfusate.CONCLUSION: In situ perfused rat intestine-liver models are suitable for the study of metabolism of chlorogenic acid in intestine and liver of rats.Chlorogenic acid was extensively metabolized in rat intestine.

Key words: in situ perfused rat intestine-liver model, chlorogenic acid, caffeic acid, ferulic acid, hippuric acid, metabolism

CLC Number:

R969.1

WANG Su-jun, ZHANG Zhi-wei, ZHAO Yan-hong, ZHANG Zhen-qing, XIE Hai-tang, RUAN Jin-xiu. Establishment of in situ perfused rat intestine-liver model for evaluation on chlorogenic acid metabolism[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(12): 1340-1344.

References

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