Effects of emodin on differentiation of bone marrow stroma cell into osteoblast in rats in vitro

Abstract

Abstract: AIM: To investigate the effects of emodin on the differentiation of MSCs into osteoblast in rats in vitro.METHODS: The MSCs were isolated from onemonth old SD rats by density-gradient centrifugation and the ALP activity and osteocalcin content were examined by PNPP and radioimmunoassay.RESULTS: Through density-gradient centrifugation isolation and subculture attachment selection, the cultured MSCs became much purified.Emodin induced the differentiation of MSCs into osteoblast by increasing ALP activity.No significant effects of emodin on osteocalcin content of MSCs were observed at concentration from 2.5×10^-7 to 10^-5 mol°L^-1. CONCLUSION: Emodin can partly induce the osteogenic differentiation.

Key words: emodin, bone marrow stromal cells, osteoblast, differentiation, rat

CLC Number:

R966

LIU Yu-yu, YAO Wei-min, AI Chun-mei, XU Bi-lian, ZOU Li-yi, CUI Liao, WU Tie. Effects of emodin on differentiation of bone marrow stroma cell into osteoblast in rats in vitro[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(2): 191-195.

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