The effect and possible mechanism of curcumin on experimental necrotizing enterocolitis

Abstract

Abstract: AIM: To observe effects of curcumin on oxidative damage in intestines of newborn rats with necrotizing enterocolitis (NEC) , as well as investigate the potential mechanism of NEC. METHODS: Twenty four Sprague-Dawley (SD) rats were randomly divided into 3 groups right after birth. Rats in the groupⅠwere subjected to formula feeding, cold stress, hypoxic-reoxygenation treatment as well as LPS intragastric administration. NEC intestinal damage was evaluated by histological scoring. Animals in group II were managed according to the same way except the intraperitoneal injection with curcumin. Rats in Group Ⅲ were delivered naturally and were dam fed. All rats were weighed every day and sacrificed on the 4th day. Proximal intestines of ileocecal junction were undertaken pathological sectioning and evaluated for pathological scoring by hematoxylin and eosin (HE) staining. The activity of intestines malonaldehyde (MDA),superoxide dismutase (SOD) and the expression of heme oxygenase-1(HO-1) in all rats were also detected respectively. RESULTS: Compared with group Ⅲ, rats in groupⅡshowed better growing development, improved physical activity and milder intestine gross characteristics than groupⅠeven after the same stress. The pathological scores were significant different among these three groups (P<0.05). Intestinal MDA concentration increased with the severity of intestine injure (P<0.05). SOD activities of group I and II decreased than that of group III. There was a significant difference in the first two groups (P<0.05). The expression of HO-1 was significant increased in groupⅠand group Ⅱ.The difference between the two groups was statistical significant (P<0.05).CONCLUSION: Curcumin could alleviate the intestinal injure in the experimental necrotizing enterocolitis rats. The potentional mechanism might be reducing intestinal oxidative damage through upregulating the expression of HO-1, decreasing MDA concentration as well as enhancing the activity of SOD.

Key words: Necrotizing enterocolitis, Curcumin, Malonaldehyde, Superoxide dismutase, Heme oxygenase-1

CLC Number:

R965.2
R722

LI Jin-chun, WEI Hong, WANG Xiao-ling, HAO Jing-mei, WEI Xiao-di. The effect and possible mechanism of curcumin on experimental necrotizing enterocolitis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(1): 17-23.

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