Protective effects of DDPH on myocardial ischemia reperfusion injury in isolated rat hearts

Abstract

Abstract: AIM: To investigate the protective effect of 1-(2,6-dimethylphenoxy)-2-(3, 4-dimethoxyphenylethylamino) propane hydrochloride (DDPH) on myocardial ischemia-reperfusion (I/R) injury in isolated rat hearts. METHODS: Myocardial ischemia-reperfusion injury models were built with Langendorff isolated perfusion technology and established by the ligation of left descending coronary artery (LAD) for 40 min and reperfusion for 120 min in isolated rat hearts.The influence of DDPH on left ventricular function, myocardial infarct size, lipid peroxidation and the ultramicrostructural changes of myocardial cells were observed. RESULTS: DDPH improved the damage of left ventricular systolic and diastolic function caused by I/R, obviously reduced myocardial infarct size, increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), decreased the content of MDA in myocardium, and lessen the degree of ultramicrostructural injury in myocardial cells. CONCLUSION: DDPH has a protective effect on myocardial ischemia-reperfusion injury in isolated rat heart, which may be related to inhibiting the formation of the oxygen free radical and subsequent lipid peroxidation.

Key words: DDPH, Isolated heart perfusion, Ischemia-reperfusion injury, Lipid peroxidation

CLC Number:

R962
R972.4

ZHANG Hong-xia, WU Yong-jie, GAO Ming-tang. Protective effects of DDPH on myocardial ischemia reperfusion injury in isolated rat hearts[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(2): 160-165.

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