The efficacy of Fructus Tribuli extract JL201 on type 2 diabetes and its potential mechanisms

Abstract

Abstract: AIM: To observe the effect of Fructus Tribuli extract (NO. JL201) on the concentration of fasting plasma glucose, blood insulin, TNF-α, IFN-β and that of TLR3, TLR4 mRNA in islet cell on spontaneous type 2 diabetes KK^Ay mice, and explore its potential hypoglycemic mechanisms. METHODS: Mice were randomly allocated into 5 groups: control group, JL201 high -dosage group (20 mg/kg BW), JL201 middle -dosage group (10 mg/kg BW), JL201 low-dosage group (5 mg/kg BW), and positive drug metformin group (400 mg/kg BW). Body weights, food and water intake and fasting plasma glucose (FPG) were measured weekly. Mice were sacrificed after 4 weeks administration to obtain the blood sample and measured the concentration of FPG and insulin (Ins), and calculated the sensitivity of Ins in mice. The concentrations of TLR2, TLR4 mRNA in Islet cell were measured using realtime-PCR method.RESULTS: Different concentration of JL201 reduced the intake of food and water respectively. In addition, the concentration of FPG decreased significantly (P<0.05 or P<0.01) and the concentration of serum Ins also lowed (P<0.05 or P<0.01). However, the sensitivity of Ins increased significantly (P<0.05) and the secretion of TNF-α, IFN-β reduced. At the same time, JL201 could decrease the concentration of TLR2, TLR4 mRNA spontaneously. CONCLUSION: JL201 has beneficial effects on hypoglycemic and strengthening the sensitivity of Ins. Besides, it also can improve polydipsia and polyphagia clinical symptoms. The potential beneficial effects may be associated with decreasing islet cell TLR2, TLR4 mRNA levels and inhibiting inflammation.

Key words: Fructus Tribuli, type 2 diebetes, efficacy, mechanism

CLC Number:

R965.2

ZHAO Bao-sheng, LI Peng-shou, ZHANG Shu-yuan, LI Chun-na, LIU Yang-yang, SHI Xiao-juan, XU Tun-hai, LIU Tong-hua. The efficacy of Fructus Tribuli extract JL201 on type 2 diabetes and its potential mechanisms[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(9): 1006-1010.

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