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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2002, Vol. 7 ›› Issue (6): 493-495.

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Effects of sodium magnesium fructose diphosphate on lactate concentration and ATPase activity of ischemic synaptosome

ZENG Fan-Xin, DONG Zhi1, ZHOU Qi-Xin1   

  1. Department of Pharmacology and Toxicology,Chongqing Pharmaceutical Research Institute,Chongqing 400061;
    1 Department of Pharmacology,Chongqing University of Medical Science,Chongqing 400016
  • Received:2002-09-03 Accepted:2002-09-11 Online:2002-12-26 Published:2020-11-20
  • Contact: ZENG Fan-Xin,male,engineer,master' s degree,engaged in neuropharmacology.Tel:023-62505956  E-mail:zfx2806@sohu.com
  • About author:DONG Zhi,male,MD,professor,engaged in neuropharmacology.

Abstract: AIM: To study the effects of sodium magnesium fructose diphosphate(FDPM)on lactate concentration and ATPase activity of ischemic synaptosome,so as to explore the protective mechanisms of FDPM on cerebral ischemia.METHODS: Synaptosome prepared from normal rat brain was cultured with oxygen and glucose deprived to establish the model of ischemic synaptosome.1.3 mmol·L-1magnesium sulfate,4.0mmol·L-1 fructose-1,6-diphosphate(FDP)and 1.3 mmol ·L-1 FDPM were incubated with the ischemic synaptosomes separately.After 60min,the lactate concentration as well as the activities of Na+-K+ATPase and Ca2+-Mg2+ATPase were detected.RESULTS: FDPM reduced the lactate concentration,raised the activities of Na+-K+ATPase and Ca2+-Mg2+ATPase of ischemic synaptosomes(p<0.05).The effects of FDPM were stronger than those of FDp(p<0.05).CONCLUSION: FDPM may protect cerebral ischemia by improving the energy metabolism,and mitigating acidosis state of brain tissue.

Key words: cerebral ischemia, sodium magnesium fructose diphosphate, fructose-1, 6-diphosphate, magnesium sulfate, synaptosome, lactate, Na+-K+ATPase, Ca2+-Mg2+ATPase

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