Ipriflavone up-regulates mRNA expression of osteoprotegerin in lumbar vertebra of ovariectomized rats

Abstract

Abstract: AIM: To build a postmenopausal osteoporotic model in rats and investigate the effect of ipriflavone on the expression of osteoprotegerin (OPG) mRNA in lumbar vertebra of ovariectomized rats, and to analyze its mechanism in the prevention of postmenopausal osteoporosis.METHODS: Forty female rats at 6 months of age were randomly allocated into 4 groups:sham-operated group, ovariectomized grouP(OVX) , OVX combined valerate estradiol group, and OVX combined ipriflavone group.Rats were sacrificed after they had been lavaged for 13 weeks.The fourth lumbar vertebra was dissected out for histomorphology and the second lumbar vertebra for measurement of mRNA level of OPG by way of reverse transcription polymerase chain reaction technique.RESULTS: The trabecular volume was significantly lower in OVX grouPrats than that in sham grouPrats, while osteoid seam was significantly higher.Valerate estradiol and ipriflavone can increase the trabecular volume and reduced osteoid seam significantly in OVX rats.To compare with sham grouPrats, the mRNA level of OPG decreased significantly in OVX rats, and valerate estrodiol and ipriflavone can up-regulate the expression level of OPG mRNA significantly.CONCLUSION: Our experiment built the postmenopausal osteoporotic model successfully.Valerate estradiol and ipriflavone can prevent postmenopausal osteoporosis by its inhibitory effect on bone turnover.OVX-induced bone loss was associated with OPG.Iprif lavone,just like valerate estradiol, perhaps has its antiresorbative effect on bone by the signal of OPG.Estrogen and ipriflavone can up-regulate the mRNA expression of osteoprotegerin in the lumbar vertebra tissue of ovariectomized rats.

Key words: postmenopausal osteoporosis, rat, osteoprotegerin, ipriflavone

CLC Number:

R965.2
R966

SHANG Min, YANG Xin, LI Ya-zhen, GU Bei, WU Qiong, LIAO Qin-ping. Ipriflavone up-regulates mRNA expression of osteoprotegerin in lumbar vertebra of ovariectomized rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(10): 1173-1176.

References

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