Determination of diclofenac sodium in dialysate of joint cavity in rats based on microdialysis and HPLC-ESI-MS and its application

Abstract

Abstract: AIM: To develop and validate a high liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) method for the quantification of diclofenac sodium in dialysate of joint cavity in rats in order to meet the requirement of collection and determination of non-steroidal anti-inflammatory drugs (NSAIDs) in target site based on microdialysis and use diclofenac sodium as paradigm drug. METHODS: A sensitive and specific liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) method using a unipolar quadrupole technology by optimizing chromatography and mass spectrometry conditions has been applied to determine the concentration of diclofenac sodium in dialysate of joint cavity in rats.Dialysate samples were separated by HPLC on a reversed phase C₁₈ column [Waters Symmetry-C₁₈ (dp 5 μm, 150 mm×2.1 mm ID)]with a mobile phase of methanol-0.1% formic acid (80∶20, V/V).RESULTS: The method was validated over the concentration range of 1.95-125 ng/mL with the low limit of quantitation of diclofenac sodium in dialysate was 1.95 ng/mL.Within-batch and between-batch precision (RSD.%) were all within 10% while matrix effect were between 99.9%-113.4%. CONCLUSION: The microdialysis technology was successfully applied in the collection of dialysate samples in joint cavity of rats, the determination method described in this paper is rapid also has a high sensitivity.The combination of two technologies were suitable for the collection of synovial samples, also will be helpful to study the pharmacokinetic profile of non-steroidal anti-inflammatory drugs (NSAIDs) in target site.

Key words: Diclofenac sodium, Microdialysis, HPLC-ESI-MS, Rat, Dialysate of joint cavity

CLC Number:

R965.2

ZHANG Jing, CHEN Yang, LI Pei, GUO Hai-fang, LIU Xiao-dong. Determination of diclofenac sodium in dialysate of joint cavity in rats based on microdialysis and HPLC-ESI-MS and its application[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(11): 1233-1239.

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