Effects of polysaccharide of gastrodia elata blume and electroacupuncture on the expressions of brain derived neurotrophic factor and vascular endothelial growth factor in dentate gyrus of cerebral ischemia rats

Abstract

Abstract: AIM: To observe the effects of polysaccharide of gastrodia elata blume (PGB) and electroacupuncture (EA)on the expression of brain derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF) in dentate gyrus (DG) of hippocampus of cerebral ischemia rats. METHODS: A total of 40 Sprague-Dawley rats were randomly divided into the normal control group, the model group, the EA group, the PGB group and the EA+PGB group, 8 in each group.The model was prepared by middle cerebral artery occlusion (MCAO). 2 week after modeling, PGB at 100 mg/kg was given to rats in the PGB group and the EA+PGB group by gastrogavage, once daily, for 2 successive weeks. EA at “Baihui” (DU20) and “Zusanli” (ST36) was performed to rats in the EA group and the EA+PGB group, lasting for 30 min, once daily, for 2 successive weeks.The expressions of BDNF and VEGF in the DG detected by immunohistochemical assay and image analysis.RESULTS: Compared with the normal control group, the expressions of BDNF and VEGF in the DG of the model group were increased (P<0.05).Compared with the model group, the expressions of BDNF and VEGF in the DG of the EA group, the PGB group and the EA+PGB group were increased significantly (P<0.05).The expressions of BDNF and VEGF obviously were increased in the EA + PGB group than those of the EA group or the PGB group (P<0.01).CONCLUSION: EA combined with PGB could significantly upregulat the expressions of BDNF and VEGF in the DG of cerebral ischemia rats, accelerate endogenous neural stem cells in the DG, besides, their effects were significantly higher than those of the PGB group or the EA group.

Key words: cerebral ischemia, dentate gyrus, polysaccharide of Gastrodia elata Blume, electroacupuncture, brain derived neurotrophic factor, vascular endothelial growth factor, neural stem cell

CLC Number:

R965.2

MIAO Hua-chun, WU Feng, DING Jian, XIONG Ke-ren. Effects of polysaccharide of gastrodia elata blume and electroacupuncture on the expressions of brain derived neurotrophic factor and vascular endothelial growth factor in dentate gyrus of cerebral ischemia rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(3): 260-264.

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