Role of ERK1/2 and p38MAPK in the expression of Nox1 in A10 vascular smooth muscle cell induced by Ang II and CGRP

Abstract

Abstract:

AIM: To explore the role of extracellular signal-regulated kinase1/2 (ERK1/2) and p38-Mitogen-activated protein kinase (p38MAPK) in expression of nicotinic amide adenine dinucleotide phosphate （NADPH）oxidase-1 (Nox1) in vascular smooth muscle cell (VSMC) induced by Angiotensin II (Ang II) and calcitonin gene-related peptide (CGRP). METHODS: Mouse-derived A10VSMC was cultured in vitro, the cell viability and the distribution of cell cycle was detected by MTT and flow cytometry, respectively; Western blot was used to determine the protein levels of ERK1/2, p38MAPK and Nox1. RESULTS: Compared with control or CGRP groups, the A10VSMC proliferation, protein levels of p-ERK1/2 and p-p38MAPK and Nox1 were elevated in the Ang II group, which were decreased by pretreatment of CGRP for 30 min. DPI (Nox1 inhibitor) also inhibited the A10VSMC proliferation, protein levels of p-p38MAPK and Nox1 but not p-ERK1/2 induced by Ang II. SB203580 (p38MAPK inhibitor) but not PD98059 (ERK1/2 inhibitor) enhanced the inhibitory effect CGRP on the cell proliferation and Nox1 expression induced by Ang II. CONCLUSION: The A10VSMC proliferation induced by Ang II is related to increase of Nox1 expression, and the inhibitory effect of CGRP on Nox1 expression induced by Ang II in the A10VSMC proliferation phases maybe mainly associated with p38MAPK activity but not the ERK1/2 activity.

Key words: angiotensin II, calcitonin gene related peptide, vascular smooth muscle cell, Nox, MAPK

CLC Number:

R965.2

ZHANG Xiaoyi, YU Xiaohua, LIU Yuhuan, YANG Li, WANG Zhen, YANG Fang, QIN Xuping, ZENG Siyu. Role of ERK1/2 and p38MAPK in the expression of Nox1 in A10 vascular smooth muscle cell induced by Ang II and CGRP[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(1): 13-19.

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