Expression of RGS4 and D2 receptor signaling pathway in striatum of methamphetamine-dependent CPP rats

Abstract

Abstract:

AIM: To study the expression of signal transduction molecules in the striatum G protein protein 4 (RGS4) and dopamine D2 receptor (D2) in conditioned place preference (CPP) rats treated with methamphetamine (meth). METHODS: METH dependence CPP model was established (1 week and 2 weeks of METH dependence groups), The protein expression of RGS4 and D2, inhibitory G protein alpha-subunit (Gαi), mitogen-activated protein kinase (MAPK) in striatum were determined by Western blotting (WB). The changes of cyclic adenosine monophosphate (cAMP) content in striatum of rats were determined by enzyme linked immunosorbent assay (ELISA). RESULTS:Compared with saline control group, the average time of rats in the methamphetamine-paired chamber for two groups was increased (P<0.05). Compared with saline control group, RGS4 protein expressions in the two METH dependent groups were reduced (P<0.01); compared with 1 week of METH dependence group, that of 2 weeks group was reduced significantly (P<0.05). D2, Gαi, MAPK protein and cAMP expressions in the two METH dependent groups were increased (P<0.01); compared with 1 week of METH dependence group, those of 2 weeks were increased significantly (P<0.05). CONCLUSION: RGS4 and D2 receptor signaling pathways in striatum have changed in METH dependent rats, RGS4 may be involved in the regulation of METH-dependent D2 receptor signaling pathway in METH dependent rats.

Key words: methamphetamine, CPP, RGS4, D2, Gαi, MAPK, cAMP

CLC Number:

R965.2

MENG Guoyi，HE Fanyi，SUI Nianhan, ZHAO Linbo, HONG Shijun, XING Yuming，ZHAO Yongna. Expression of RGS4 and D2 receptor signaling pathway in striatum of methamphetamine-dependent CPP rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(12): 1377-1381.

References

［1］UNODC. World Drug Report 2012［M］. United Nations publication, Sales No. E.12.XI.1, 2012
［2］Grant KM, Levan TD, Wells SM, et al. Methamphetamine-Associated Psychosis［J］. J Neuroimmune Pharmacol, 2012, 7(1):113-139.
［3］Maryam R, Effat R, Nasrin H, et al. Effect of electrical stimulation of nucleus accumbens with low, median and high currents intensities on conditioned place preference induced by morphine in rats［J］. Adv Biomed Res，2014, 3:14.
［4］Wise RA. Addiction becomes a brain disease［J］. Neuron, 2000, 26(1):27.
［5］Chan AS, Yeung WW, Wong YH. Integration of G protein signals by extracellular signal-regulated protein kinases in SK-N-MC neuroepithelioma cells［J］. J Neurochem，2005, 94(5):1457-1470.
［6］Mizoguchi H, Yamada K, Mizuno M, et al. Regulations of methamphetamine reward by extracellular signal-regulated kinase 1/2/ets-like gene-1 signaling pathway via the activation of dopamine receptors［J］. Mol Pharmacol， 2004, 65(5):1293-1301.
［7］Owens WA, Williams JM, Saunders C, et al. Rescue of dopamine transporter function in hypoinsulinemic rats by a D2 receptor-ERK-dependent mechanism ［J］. J Neurosci, 2012, 32(8):2637-2647.
［8］Mirnics K, Middleton FA, Stanwood GD, et al. Disease-specific changes in regulator of G-protein signaling 4 (RGS4) expression in schizophrenia［J］. Mol Psychiatry, 2001, 6(3):293-301.
［9］Paxinos G, Watson C. The rat brain in stereotaxic coordinates fourth edition［J］. Rat Brain Stereotaxic Coordinates, 2009, 3(2):6.
［10］Kim G, Jung S, Son H, et al. The GABAB receptor associates with regulators of G-protein signaling 4 protein in the mouse prefrontal cortex and hypothalamus［J］. Bmb Reports, 2014, 47(6):324-329.
［11］刘建新, 王小亚, 王晓峰,等. 扣带回毁损对甲基苯丙胺大鼠颞叶皮质多巴胺D2受体的影响［J］. 国际神经病学神经外科学杂志, 2006, 33(3):211-213.
［12］洪仕君, 李俊麟, 李利华,等. 吗啡依赖鼠成瘾相关脑区腺苷酸环化酶的变化［J］. 法医学杂志, 2006, 22(4):254-257.
［13］Schroeder JA, Hummel M, Unterwald EM. Disruption of G i/o, protein signaling in the nucleus accumbens results in a D1 dopamine receptor-mediated hyperactivity［J］. Pharmacol Biochem Behav，2004, 79(1):93-99.
［14］Valjent E, Corvol J, Pages C, et al. Involvement of the extracellular signal-regulated kinase cascade for cocaine-rewarding properties［J］. J Neurosci, 2000, 20(23):8701-8709.
［15］Velazquez EE, Valdomero A, Maldonado NM, et al. Perinatal protein deprivation facilitates accumbal ERK phosphorylation in cocaine-sensitized adult rats［J］. Behav Brain Res, 2013, 241(1):222-227.
［16］Lerner TN, Kreitzer AC. RGS4 is required for dopaminergic control of striatal LTD and susceptibility to parkinsonian motor deficits［J］. Neuron, 2012, 73(2):347-359.
［17］Min C, Cheong SY, Cheong SJ, et al. RGS4 exerts inhibitory activities on the signaling of dopamine D2 receptor and D3 receptor through the N-terminal region［J］. Pharmacol Res, 2012, 65(2):213-220.

[1]	LIU Nanyu, YUE Hongmei, SONG Peipei, WEI Jifang, WEI Yaqian, XIE Yingying, WANG Jiaqi . PDE4 inhibitors serve as therapeutic targets for pulmonary fibrosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(3): 355-360.
[2]	ZHU Ruifang, GUO Dongkai, ZHI Hui, JIANG Yiguo, ZHANG Yueling, QIAN Xiaoping, JI Shiliang. Exploring the mechanism of elemene synergistic bortezomib against multiple myeloma by ROS-NF-κB-p38MAPK pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1219-1226.
[3]	JIN Yiyi, ZHOU Keting, YANG Chengcheng, XU Ping, ZHU Suyan. Osthole attenuates diabetes-induced renal injury by regulating NF-κB and p38/MAPK pathway mediated inflammatory responses [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(6): 622-631.
[4]	TAN Fenglang, REN Hongli, WANG Hongfei. Effect of Phellodendri amurense extract on hippocampal nerve cells induced by Aβ_1-42 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1213-1220.
[5]	YAN Pan, SHI Jianfei, LI Jing, WANG Shengdong, WANG Shuqi, WANG Chengpeng, SONG Mingfen. Effects of DRD2 and 5-HTR2A gene polymorphisms and their interaction on olanzapine in the treatment of schizophrenia [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(10): 1131-1138.
[6]	QUAN Haiyan, LIU Yuhuan, YANG Li, YANG Fang, QIN Xuping. Improvement of CGRP on vascular endothelial cells insulin resistance via activation of cAMP/PPARγ/eNOS pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(6): 615-622.
[7]	DAI Lei, LUO Linghe, WU Liyan, WANG Gang, FEI Baoying. Expression of miR-222 and MBD2 in gastric cancer patients and their effects on platinum chemosensitivity [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(6): 623-629.
[8]	LI Min, WANG Shanshan, LIU Zhonghe, JIANG Deguo. Expression and mechanism of p38MAPK signaling pathway in chronic pulpitis rats with pain [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(6): 644-649.
[9]	DU Tianping, MENG Zudong. Mechanism of 17β-estradiol on p38MAPK signal transduction pathway in propofol-induced hippocampal neuronal apoptosis in developing rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(2): 128-133.
[10]	BAI Xuemin, ZOU Likao, JIN Zhenmu, JIANG Lijia. Effects of N-acetylcysteine on regulation of EGFR/MAPK signaling pathway in airway mucus hypersecretion in chronic obstructive pulmonary disease [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(10): 1120-1127.
[11]	YAO Xinming, KONG Xiang, ZHAO Yongli, HUA Qiang, HE Chunling, YU Dan, LI Yeqiong, DAI Xiaokang, YE Shandong. Metformin inhibits p38MAPK signaling pathway and oxidativestress in rat glomerular mesangial cells cultured with high glucose [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(6): 614-620.
[12]	CHENG Xiaolong, HU Kunmei, HU Haoran，XU Hanghang, YANG Jieren, HAN Jun. Effects of Chinese medicine Yuxia capsule on renal fibrosis in spontaneously hypertensive rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(6): 632-639.
[13]	ZHAO Linbo，SUI Nianhan，LI Lihua，HONG Shijun, XING Yuming, ZHAO Yongna. Methamphetamine dependent influenced RGS4 and mGluR5 receptor signaling in striatum of rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(5): 536-540.
[14]	YANG Yi，GUAN Qiaobin，GUO Li，HAN Chenyang. Antrodia camphorata polysaccharide improved neurobehavioral function in Parkinson mice via reducing the expression of NLRP3-Caspase1 inflammatory body [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(4): 406-412.
[15]	ZHANG Rui, LI Bingjie, LI Qinglin. Jujuboside A attenuates LPS-induced microglial activation and provides neuroprotection [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(2): 126-131.