Abstract:

AIM: To study the effect of resveratrol  (RES) on the expression of Wnt/β-Catenin signaling pathway in ovariectomized osteoporosis rats, and to investigate the protective effect and its possible mechanism.  METHODS: Sixty female SPF rats were randomly divided into sham operation group (Sham, n=12) and ovariectomized group (OVX, n=48). Ovariectomy was performed to establish the ostoporotie model. The bone density (BMD) was detected after 12 weeks to assure the successful modeling. OVX group were thus randomly divided into OVX group, resveratrol low (RES-L, 5 mg·kg-1·d-1), resveratrol middle (RES-M, 15 mg·kg-1·d-1) and high dose group (RES-H, 45 mg·kg-1·d-1), with twelve rats in each group receiving gavage, while the sham operation group and model group were given normal saline of the same dose. Drug intervention was performed once a day and lasted for 12 weeks. Levels of serum progesterone (P) and estradiol (E2) and expression of tartrate-resistant acid phosphatase (TRAP) were detected by ELISA method; BMD was measured; Expression of β-Catenin, glycogen synthasc kinase-3β (GSK-3β), low-density lipoprotein receptor-related protein 5 (LRP5) and Runx2 mRNA was detected by RT- PCR method. RESULTS:The BMD of OVX group was significantly lower than that of the Sham group (P<0.05), which suggested successful modeling; after drug intervention for 12 weeks, BMD of the OVX group decreased significantly (P<0.05), while that of the RES groups increased significantly compared with Sham group; compared with the OVX group, the expression of BMD, β-Catenin, LRP5 and Runx2 mRNA in RES groups increased significantly (P<0.05); the expression of GSK-3β mRNA decreased significantly(P<0.05).CONCLUSION: Res can significantly improve the BMD of ovariectomized rats, and the mechanism is related with up-regulating the Wnt/β-Catenin signaling pathway.

Key words: resveratrol, osteoporosis, Wnt/β-Catenin