Effects of curcumin on proliferation of VSMCs and expression of PTEN induced by PDGF-BB

doi:10.12092/j.issn.1009-2501.2019.12.007

Abstract

Abstract:

AIM: To study the effects of curcumin on the proliferation of vascular smooth muscle cells (VSMCs) and the deletion of phosphatase and tensin homology deletedon chromosome ten (PTEN) induced by platelet-derived growth factor-BB (PDGF-BB) and to provide more abundant experimental basis for the clinical application of Dong nationality medicine "Curcuma longa". METHODS: The proliferation model of VSMCs was established by incubating with PDGF-BB (20 ng/mL) for 24 h. The cell activity was observed by MTT and CCK-8, cell cycle and cell migration were detected by flow cytometry and cell scratch assay, respectively. The effects of PTEN mRNA, PTEN protein and phosphorylated PTEN (p-PTEN) protein were observed by RT-PCR and Western blot. RESULTS:10, 30 μmol/L of curcumin was significantly inhibited the proliferation and migration of VSMCs induced by PDGF-BB (20 ng/mL). 10 μmol/L curcumin was used as the optimum concentration, it could not only increase the ratio of G0/G1 phase and decrease the proportion of S phase, but also inhibit the migration of VSMCs. At the same time, it could up-regulate the expression of PTEN mRNA and PTEN protein, down-regulate the expression of p-PTEN protein. CONCLUSION: Curcumin can inhibit the proliferation and migration of VSMCs induced by PDGF-BB. The effect of curcumin on PTEN may be related to the up-regulation of PTEN mRNA and PTEN proteins, down-regulation of p-PTEN proteins.

Key words: curcumin, platelet-derived growth factor-BB, vascular smooth muscle cells, phosphatase and tensin homology deletedon chromosome ten

CLC Number:

R965.2

QIU Fei, GAO Dan, YANG Dongmei, ZHONG Xiaomei, TANG Sha, YIN Huiming, ZHANG Zaiqi. Effects of curcumin on proliferation of VSMCs and expression of PTEN induced by PDGF-BB[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(12): 1358-1363.

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