Abstract: Objective To explore correlation of ADAM33 V4 gene polymorphism and vitamin D (Vit D) level with asthma of children in Urumqi. Method Totally 154 asthmatic children and 120 healthy non-asthmatic children aged 3~15 in Urumqi were selected.ADAM 33 V4 gene single nucleotide polymorphism loci (SNPs) genotyping were detected using PCR polymerase chain reaction and VitD levels were detected by ELISA.Further stratified according ethnic groups were performed and correlation of ADAM33 V4 gene polymorphism and VitD level with asthma were analyzed. Result 1)V4 sites of the frequency distribution of three genotypes between cases and control groups were statistically significant differences (χ²=6.806,P=0.033) and,it showed no significant difference among the different ethnic groups (case group:χ²=0.714,P=0.699;control group:χ²=5.898,P=0.052).2)VitD levels in the case group and the control group were statistically different(t=2.341,P=0.020). Conclusions 1) ADAM33 V4 gene loci is correlated with childhood asthma morbidity in children in Urumqi,but there is no difference among nationalities.V4 site after the G point mutation may be a risk factor of bronchial asthma.2) VitD levels is correlated with childhood asthma morbidity in Urumqi.

Key words: asthma, ADAM33, V4 loci polymorphism, vitamin D, Urumqi, children

摘要： 目的 探讨乌鲁木齐地区支气管哮喘儿童ADAM33基因V4位点多态性、维生素D(VitD)水平及其相关性研究。方法 选取同期3~15岁于乌鲁木齐地区生活的哮喘患儿154人,同时设立对照组健康非哮喘儿童120人,首先采用PCR聚合酶链反应对ADAM 33基因V4位点进行单核苷酸多态性(SNP)分型,并用ELISA法检测VitD水平,进一步根据民族进行组内分层,进行V4位点、VitD分析并进行相关性分析。结果 1)V4位点3种基因型频率分布在病例组和对照组间差异有统计学意义(χ²=6.806,P=0.033),在不同民族间差异无统计学意义(实验组:χ²=0.714,P=0.699;对照组:χ²=5.898,P=0.052)。2)VitD水平检测病例组与对照组比较,差异有统计学意义(t=2.341,P=0.020)。结论 1)ADAM33 基因V4位点与乌鲁木齐地区支气管哮喘儿童发病有相关性,但无民族差异。V4 位点突变后的 G 基因可能是支气管哮喘发病的危险因素。2)VitD水平与支气管哮喘儿童发病有相关性。

关键词: 支气管哮喘, ADAM33基因, V4位点, 维生素D, 乌鲁木齐, 儿童