Protective effects of 9-(4-ethoxycarboxylyphenoxy)-6,7-dimethoxy-1,2,3,4-tetrahydro acridine on anoxia and ischemic injury in cultured PC12 cells

Abstract

Abstract: Aim To investigate the effects of EDT on anoxia and ischemic injury in cultured PC12 cells.Methods Cultured PC12 cells were treated with 1 mmol·L^-1 Na2S2O4 and 20 mmol·L^-1 NaCN in combination with glucose deprivation.The pro tective effects of EDT on these two models were evaluated by lactate dehydrogenate (LDH) ef flux assay and colormetric MT T assay.Results EDT, within the range of 10^-8～10^-6 mol·L^-1, significantly antagonized LDH efflux induced by two models and increased the optical density at 570 nm tested by colorimetric MTT assay in concent ration-dependent manner.10^-6 mol·L^-1 EDT might time-dependently inhibit two injuries and reach maximal level at 48 h.Conclusion EDT can protect PC12 cells from anoxia and ischemic injury.

Key words: 9-(4-Ethoxy carbo xylyphenoxy)-6,7-dimethoxy-1, 2, 3, 4-tetrahydro acridine, PC12 cultured cell, anoxia injury, ischemic-like injury

CLC Number:

R966

SHENG Rui, LIU Guo-Qing. Protective effects of 9-(4-ethoxycarboxylyphenoxy)-6,7-dimethoxy-1,2,3,4-tetrahydro acridine on anoxia and ischemic injury in cultured PC12 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2001, 6(4): 330-333.

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