Abstract: AIM: To investigate the antio xidation of proanthocy anidins from grape seeds (GSPE)on 2, 4, 6-t rinitrobenzene sulfonic acid (TNBS)twice-induced recurrent ulcerative colitiss (UC)in rats and to probe into its underlying mechanism.METHODS: Recurrent colitis model was established in Wistar male rats by rectal administ ration of 80 mg/kg TNBS dissolved in 50 %ethanol, and then the rats were second instilled with 30 mg/kg TNBS into the colon on the 16th day after the first induction UC.Rats were intragastrically administered different doses of GSPE (100, 200, and 400 mg/kg)per day for 7 days after twice-induction of colitis by TNBS. Sulfasalazine (SASP, 500 mg/kg)was used as a positive control drug.Rats were killed after GSPE treatment 7 days.The colon sample was extracted, and the colon weight/length ratio (mg/cm)was assessed.The activities of, myeloperoxidase (MPO), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), inducible nitricoxide synthase (iNOS), and the contents of malony ldialdehy de (MDA)and glutathione (GSH)and NO in se rum were detected by biochemistry method.RESULTS: Compared with the model group, the rat body w eight was decreased, and the colonic weight/length ratio was reduced in GSPE dosage group(P <0.05 or P <0.01);the activities of MPO and iNOS and the contents of MDA,NO in serum were significantly decreased(P <0.05 or P <0.01);but the activit ies of MPO, iNOS and the contents of MDA, NO were decreased (P <0.05 or P <0.01);the activities of SOD, GSH-Px, the contents of GSH in serum were increased(P <0.05 or P <0.01).CONCLUSION: GSPE can alleviate the inflammatory reactions in colitisthrough inhibiting oxygen free reaction, exerting antioxidation effects and decreasing the production of NO.

Key words: Proantho cyanidins from grape seeds, 2,4,6-trinit robenzenesulfonic acid, Current, Ulcerative colitis, Anti-inflammatory, Mechanism