Experiment study of Qing-ying-xie-yu Fang (QXF) in the treatment of intestinal ischemia-reperfusion in rats

Abstract

Abstract: AIM: To investigate the protective effects and possible mechanisms of Qing-yingxie-yu Fang (QXF) in the treatment of intest inali schemia-repe rfusion in rats. METHODS: Forty Sprague Daw ley rats were randomly divided into sham(n =8), model (n =16) and QXF(n =16) groups. The last two groups were divided into 2 hours g roup and 24 hours group after reperfusion respectively. Intestinal ischemia-reperfusion model in rats was induced by clamping the superior mensenteri cartery for 45 minutes. The pathologic injury of the intestines and the levels of TN F-α, IL-10, D lactic acid in blood were examined at 2 hours point and 24 hours point after reperfusion respectively. The statistical analysis was finished with SPSS 11.0 software. RESULTS: Intestinal ischemia-reperfusion model in rats was successfully induced by clamping the superior mensen tericartery for 45 minutes. QXF alleviated the patho logic injury of the intestines, and reduced the levels of TN F-α, Dlactic acid (P<0.05) in the early period and the level of IL-10 (P<0.05) in the late period. CONCLUSION: QXF can protect the intestines of rats from the injury of intestinali schemia-reper fusion. The following is the possible mechanisms: to clear up dunghill in intestines, regulate the balance of inflammatory mediators and protect gut barrier function.

Key words: Intestine ischemia-reperfusion injury, Qing-Ying-Xie-Yu Fang, Gut barrier function, Rat

CLC Number:

R965.2

WU Zhi-ming, CHEN Jiang, CHU Xiu-feng, LOU Jian-ping, MENG Xing-cheng, QIUHai-jiang. Experiment study of Qing-ying-xie-yu Fang (QXF) in the treatment of intestinal ischemia-reperfusion in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(3): 282-286.

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