Abstract: AIM: To study the anti-oxidative effects of Radix Angelica and Rhizoma Ligusticum with different ratio extracted by water, ethanol, alcohol-water. METHODS: Hydroxyl radical generated in Fenton reaction system and oxidative insult in HUVEC induced by H₂O₂ were used in the present study. RESULTS: Water preparation exhibited the strongest scavenging activity against OH generated in a Fenton reaction system. Ethanol preparation had the weakest activity against OH, while exerted strongest activity on damaged HUVEC. Water preparation (1∶1), ethanol preparation (1∶1.5) and alcohol-water preparation (2∶1) were found to be the most potent. CONCLUSION: Radix Angelica and Rhizoma Ligusticum with different ratio exert markedly different anti-oxidative activities. The most reasonable ratio may appear 1∶1 for water preparation and be consistent with clinical ratio in si-wu-tang, a famous TCM fomulae.

Key words: Radix Angelica, Rhizoma Ligusticum, Combination, Ratio, Anti-oxidative effect