Effect of sequoyitol on lipid metabolism in type 2 diabetic rats

Abstract

Abstract: AIM: To observe the effects of sequoyitol on lipid metabolism in rats with type 2 diabetes.METHODS: Type 2 diabetic rats were induced by fed with a high fat and high sugar diet as well as injected with a low dosage of streptozotocin (35 mg/kg). Sequoyitol (12.5, 25, 50 mg·kg^-1·d^-1) was orally administered once a day in 35 model rats for 6 weeks. The contents of TG, TC, and low density lipoprotein cholesterol (LDL-C) were measured using fully automatic biochemical analyzer. ELISA was used to measure the contents of free fatty acids (FFA) in plasma,and the levels of apolipoprotein A₁ (Apo-A₁) and apolipoprotein B₁(Apo-B₁) in liver tissues. The content of leptin was measured with radioimmunoassay. In addition,pathological changes in liver was observed with MASSON staining.RESULTS: Sequoyitol reduced the contents of blood lipids,FFA,Apo-B₁ and leptin,elevated the content of Apo-A₁. At the same time,sequoyitol ameliorated hepatocellular fatty degeneration, reduced collagen deposition at liver cell interstitial and next to the blood vessels.CONCLUSION: The results show that sequoyitol regulates lipids metabolism and inhibites hepatic steatosis and collagen deposition in type 2 diabetes rats.

Key words: Sequoyitol, Type 2 diabetes, Rat, Blood lipids , Apolipoprotein, Leptin

CLC Number:

R965.2

CHEN Xiang-pan, YANG Jie-ren, LIU Yan, HAO Wei, LI Xian-wei. Effect of sequoyitol on lipid metabolism in type 2 diabetic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(6): 633-637.

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