Effects of electroacupuncture combined with compound salviae miltiorrhizae on the expressions of BDNF, TrkB and VEGF in cortex of chronic cerebral ischemia rats

Abstract

Abstract: AIM: To observe the effects of electroacupuncture (EA) combined with Compound Salviae Miltiorrhizae (CSM) on the expressions of brain derived neurotrophic factor (BDNF),Tyrosine kinaseB(TrkB) and vascular endothelial growth factor (VEGF) in cortex of chronic cerebral ischemia rats,and to explore its mechanism of treating chronic cerebral ischemia.METHODS: Sprague-Dawley rats were subjected to permanent bilateral Ligation of common carotid artery to prepare chronic cerebral ischemia model rats. The 32 rats were successfully made and were randomly divided into model group,CSM group,EA group,and EA+CSM group(n=8). One week after modeling,CSM at 0.75 g/kg was given to CSM group rats by gastrogavage,EA at Baihui and Dazhui was performed to EA group rats for 30 min,the EA+CSM group was treated with both CSM gastrogavage and electroacupuncture therapy,once a day for 5 weeks. In the experiments, eight SD rats were used as control group.The expressions of BDNF,TrkB and VEGF in the cortex area were detected by immuno-histochemical assay and image analysis.RESULTS: Compared with the normal control group,the expressions of BDNF,TrkB and VEGF in the cortex of the model group were obviously lower (P<0.01 or P<0.05). Compared with the model group,the expressions of BDNF,TrkB and VEGF in the cortex of the CSM group,the EA group,and the EA+CSM group were obviously increased (P<0.01 or P<0.05). The BDNF,TrkB and VEGF expressions in the cortex of EA+CSM group was obviously higher than those of the CSM group and the EA group (P<0.01 or P<0.05).CONCLUSION: EA combined with CSM can protect the chronic cerebral ischemia rats from the ischemia injury,the mechanism of which may related to up-regulating the expressions of BDNF,TrkB and VEGF.

Key words: Compound salviae miltiorrhizae, Electroacupuncture, Cerebral ischemia, BDNF, TrkB, VEGF

CLC Number:

R965.2

ZHANG Ye-gui, DIN Yan-xia, HOU Liang-qin. Effects of electroacupuncture combined with compound salviae miltiorrhizae on the expressions of BDNF, TrkB and VEGF in cortex of chronic cerebral ischemia rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(4): 376-381.

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