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Tracing study on the neurobehavioral effects of neonatal rats by the intrauterine human cytomegalovirus infection.
- PAN Na-na, WANG Li-li, WU De, TANG Jiu-lai.
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2014, 22(6):
600-604.
DOI: 10.11852/zgetbjzz2014-22-06-13
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Objective To detect the dynamic influence of nervous system development in neonatal rats by the intrauterine human cytomegalovirus (HCMV) infection. Methods The SD rat model of intrauterine HCMV infection was established, then the rats selected from experimental group and the control group randomly were done neuroethology test, such as Morris water maze test, hanging test, slope test, the open field test etc at the age of 1, 3 and 6 months respectively.Before and after the experiment, the light microscope and electron microscopy were used to observe their dynamic pathological change of brain tissue separately. Results Compared with normal control group, the Morris water maze escape latency, swimming distance of the experimental group at the age of 1, 3, 6 months were significantly higher than those of the control group, at the same time cross the platform number was significantly lower than control group, and with the increase of age, the cross platform ability was drop obviously (F=45.09, P<0.01);Motor functionrats' hanging test scores were (1.49±0.53), (1.23±0.46), (0.93±0.35) in infected group at the age of 1, 3 and 6 months, while those in the control group were (2.29±0.49), (2.14±0.69), (1.98±0.25), there were significant differences between two groups(samples nonparametric test, P all<0.001), suggested sport function of infected neonatal rat was decreased significantly.Open filed resultoffspring's across the grid number were (44.0±7.32), (37.40±9.56), (26.62±8.87) in infected group in 1, 3 and 6 months of age, while those in the control group were (57.07±9.65), (55.27±9.36), (54.27±8.14), there were significant differences between two groups (P all <0.05).Pathological results showed that along with the age growth, the hippocampus structure and nerve myelin of the experimental group offspring were aggravate gradually. Conclusion Intrauterine HCMV infection may result in the abnormal neural behavior of neonatal rat, and the impairment of learning and memory, motor function and emotional behavior development is aggravate gradually.