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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2005, Vol. 10 ›› Issue (7): 750-754.

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Design of livin antisense oligonucleotides with computer

ZHANG Hua-dong, YUAN Shou-jun, CHEN Hui-peng   

  1. Department of Pharmacology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China
  • Received:2005-04-26 Revised:2005-06-23 Online:2005-07-26 Published:2020-11-10

Abstract: AIM: Antisense oligonucleotides against livin were designed with computer software. METHODS: Antisense oligonucleotides were designed according to the secondary structure of livin mRNA which was simulated with RNAdraw and Sfold. And then these oligonucleotides were transfected into Hela cells for inducing apoptosis. RESULTS: Five antisense oligonucleotides were designed using RNAdraw and Sfold, and effectively induced Hela cell apoptosis. CONCLUSION: The approach is effective and feasible to design antisense oligonucleotides by means of calculation with two kinds of software.

Key words: the secondary structure of mRNA, RNAdraw, Sfold, livin antisense ODNs

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