Effects of curcumin pretreatment on heme oxygenase-1 in lung injury induced by ischemia-reperfusion of hind limbs in rats

Abstract

Abstract: AIM: To investigate the effects of curcumin pretreatment on heme oxygenase-1 (HO-1) in lung injury induced by ischemia-reperfusion (I/R) of hind limbs in rats.METHODS: The model of lung injury was established by occlusion of bilateral femoral arteries for 2 h followed by 3 h reperfusion in rat. Sixty SD rats were randomly divided into five equal groups: Sham group, Curcumin group (Cur group), I/R group, Curcumin+I/R group (Cur-I/R group), Zinc protoporphyrin IX+Curcumin+I/R group (ZnPP-Cur-I/R group). ZnPP-Cur-I/R group received ZnPP 20 mg/kg via intraperitoneal injection 24 h before ischemia. Cur, Cur-I/R and ZnPP-Cur-I/R groups received curcumin 200 mg/kg respectively via intraperitoneal injection 2 h before ischemia. I/R of hind limbs was induced in three I/R groups. The lungs were removed for pathologic examination and determination of the lung Wet/dry weight ratio (W/D), lung injury score and polymorphonuclear neutrophil (PMN) count. The expression of HO-1 mRNA and protein in lung tissues was detected using RT-PCR and Western blot analysis respectively.RESULTS: Alveolar edema, localized pulmonary atelectasis and large amount of PMN infiltration were found in I/R group and were ameliorated in Cur-I/R group. Lung injury score, W/D ratio and PMN count were increased in I/R group as compared with Sham group (P＜0.01). Lung injury score, W/D ratio and PMN count were significantly decreased in Cur-I/R group as compared with I/R group (P＜0.01). HO-1 mRNA and protein expression was significantly up-regulated in Cur-I/R group than in Sham and I/R groups (P＜0.01). Compared with Cur-I/R group, the beneficial effects of curcumin were abolished, lung injury score, W/D ratio and PMN count were significantly increased, and expression of HO-1 mRNA and protein was down-regulated in ZnPP-Cur-I/R group (P＜0.01).CONCLUSION: Curcumin pretreatment has protective effects against lung injury induced by I/R of hind limbs in rats through up-regulation of HO-1 expression in the lung tissues.

Key words: Curcumin, Reperfusion injury, Heme oxygenase-1

CLC Number:

R563

SUN Xiao-feng, YANG Jun. Effects of curcumin pretreatment on heme oxygenase-1 in lung injury induced by ischemia-reperfusion of hind limbs in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(8): 841-845.

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