Table of Content

Volume 14 Issue 2
26 February 2009

Previous Issue    Next Issue

Research progress in transfactor PITX2

WANG Guo, LI Zhi, ZHOU Hong-Hao

2009, 14(2):  121-125. 

Asbtract ( 92 )   PDF (223KB) ( 146 )  

References | Related Articles | Metrics

PITX2 is a member of bicoid transcriptional factor family.The PTX subfamily is composed by PITX2 and two other homologous proteins, PITX1 and PITX3.This article reviews the structure, expression and function of PITX2, the relationship between PITX2 andWnt β-catenin signal pathway.Meanwhile, the genetic diseases resulted by mutations of PITX2 and the molecular mechanisms have also been reviewed.

Screening of immuno-regulatory components in extract of Yu-ping-feng powder based on delayed type hypersensitivity and Th cytokines

WANG Liang, HONG Min, BIAN Hui-min, ZHENG Jie, KUANG Ye, DUAN Jin-ao

2009, 14(2):  126-130. 

Asbtract ( 68 )   PDF (241KB) ( 139 )  

References | Related Articles | Metrics

AIM: To screen immunoregulatory components by evaluating the effects of different extracts of Yu-ping-feng powder on allergic contact dermatitis (ACD)which is a well known animal model for delayed type hypersensitivity(DTH).Proliferation of T lymphocytes and secretion of the cytokines induced by ConA were also applied to evaluate the effects of extracts of Yu-ping-feng powder.METHODS: Mice ACD were induced by DNCB and the proliferation of T lymphocytes was measured by MTT.The levels of cytokines were determined by ELISA.RESULTS: The swelling degree of mice auricle was reduced significantly by the alcohol extract substance.The T cell proliferation and IFN-γ IL-4 secretion stimulated by ConA were inhibited significantly by this site.Significant effects were also found in the acetic ether extract parts treated groups including reduction of DTH in vivo, inhibition of IFN-γproduction and increase of IL-4 level in vitro.The ligarine extract part was found to inhibit IFN-γand IL-4 secretion in vitro while no significant effect was detected in mice model.As far as the IFN- γ IL-4 ratio was concerned, the alcohol extract part, the ligarine extracting site and the acetic ether extracting site had certain inhibitory action.CONCLUSION: The active components for DTH are wherein the alcohol extracting site and the acetic ether extracting site of Yu-ping-feng powder and their effects were related to the regulation of Th1 Th2 balance.At the same time the results indicate that it is reasonable for Yu-ping-feng used as powder in clinic.

Effects of N-acetyl-L-cysteine on promoting permeation and transport of insulin across the buccal TR146 cell culture model

XUE Xiao-yan, JIA Min, SUN Yang, ZHOU Ying, TANG Jian, CHEN Ying-ying, LUO Xiao-xing

2009, 14(2):  131-135. 

Asbtract ( 93 )   PDF (201KB) ( 85 )  

References | Related Articles | Metrics

AIM: To study the effects of N-acetyl-L-cysteine (NAC)on permeation and transport of insulin across the buccal TR146 cell culture model. METHODS: A human buccal epithelium model was established using TR146 cell line grown on Millicell culture plate inserts.The effects of NAC (1.25 %, 2.5 %, 5 %, 10 %)on permeation and transport of insulin across TR146 multilayer were studied.The apparent permeability coefficient (Papp)of insulin and the enhancement ratio (ER)of NAC were calculated, and the effects of NAC (1.25 %, 2.5 %, 5 %, 10 %) on TR146 cell activity were studied with MTT assay. RESULTS: 5 %, 10 % NAC significantly enhanced the permeation and transport of insulin across TR146 multilayer.Their Papp were 4.92 ±0.62 and 5.04 ± 0.52 (×10 ^-6 cm s, n =5) respectively, and ER were 5.22 ±0.88 and 6.05 ±0.18 respectively (n = 5), all of which were statistically higher than that of control group (P <0.05).The cell relative enzyme activity was significantly decreased, and those in the other groups had no significant defference compared with control group.CONCLUSION: 5 % NAC can greatly enhance the permeation and transport of insulin across TR146 multilayer, with little toxicity effects on the activity of TR146 cells.Therefore, NAC would be one safe and effective permeation enhancer for insulin transport.

Protective effect of epidermal growth factor on the atrophic gastric mucosa in rats

YUAN Qing-feng, SI Jian-min, ZHOU Wen, ZHANG Xiao, ZHANG Xiao-feng, LV Wen

2009, 14(2):  136-139. 

Asbtract ( 68 )   PDF (240KB) ( 157 )  

References | Related Articles | Metrics

AIM: To explore the protective effect of epidermal growth factor(EGF)on the atrophic gastric mucosa in rats.METHODS: The rats of established models of atrophic gastric mucosa were divided into 3 groups.There were treated by EGF subcutaneous injection, sucralfate by mouth and saline subcutaneous injection respectively.12 weeks later rats were executed with aether and the stomachs were removed.The gastric streak-like samples for histological examination were cut and attained with hematoxylin and eosin(HE) and with immunohistochemistry for proliferation cell nuclear antigen(PCNA)to observe a series of indexes as follows :Ratios of the thickness of gastric mucosal glands and muscularis mucosa(L1 L2);The number of gastric glands in 1 mm lengths of mucosal layer in longitudinal sections;The length of the proliferation zone represented with the length from top to bottom of PCNA positive cells.RESULTS: In the therapy groups, the gastric glands were rearranged in order and without sings of malignant proliferation.The means of L1 L2 in three groups were 8.6 ±1.2, 6.6 ±1.6 and 5.0 ±0.9 respectively.EGF significantly increased the ratios of L1 L2 than other groups(P <0.05, P <0.01).The number of gastric glands in 1 mm length of mucosal layer were 52.0 ±5.8, 34.8 ±4.3, 27.1 ±2.2 respectively.There were more number of gastric glands in EGF group and sucralfate group than that in model group (P <0.01).The means of the length from top to bottom of PCNA-positive cells were (84.5 ±2.9) μm, (76.8 ±1.7)μm and (63.3 ±9.2)μm respectively.There were more length in EGF group and sucralfate group than that in model group(P <0.05, P <0.01).CONCLUSION: EGF plays an important role in improving the gastric mucosal lesions of the rats with CAG and its effect is better than sucralfate.

Protectve effects of recombinant human tumor necrosis factor receptor: Fc fusion protein on acute lung injury induced by lipopolysaccharide in rats

WANG Shi-ting, GUO Zhu-ying, XU Mang-hua, JIAO Qiang, GAO Feng-hou

2009, 14(2):  140-143. 

Asbtract ( 62 )   PDF (271KB) ( 84 )  

References | Related Articles | Metrics

AIM: To investigate the mechanisms and protective effects of recombinant human tumor necrosis factor receptor :Fc fusion protein (rhTNFR:Fc) on the acute lung injury induced by lipopo1ysaccharide (LPS)in rats.METHODS: The rat model of acute lung injury was established with LPS and the rats were randomly divided into four groups :control group, rhTNFR: Fc group, LPS group and rhTNFR:Fc +LPS group.After administration, the rats'death was recorded, the pulmonary wet dry weight ratios were determined, the changes of lung histopathology were observed by HE dyeing, the levels of TNF-αand the bioactivity in serumwere detected.RESULTS: Compared with control group the rats'mortality rate and pulmonary wet dry weight ratio were significantly higher in the LPS group (P <0.05).The pathological examination showed lung interstitial hyperemia, oedema, and generous inflammatory cell infiltrate.The levels of TNF-αand the bioactivity in serum increased significantly (P <0.05).The mortality rate decreased and the lung pathology injury obviously ameliorated in rhTNFR :Fc +LPS group.The TNF-αbioactivity in serum decreased significantly (P <0.05)compared with the LPS group.CONCLUSION: rhTNFR :Fc could degrade TNF-αbioactivity and abate the acute lung injury induced by LPS in rats by binding TNF-α.

Protection of noninvasive delayed limb ischemic preconditioning on cortex against cerebral ischemia reperfusion injury in rats

YUAN Heng-Jie, , LUO Qiong, ZHU Xue-hui, YU Pei, LI Yu-mei, WU Yan-na, KANG Yi, JIAO Jian-jie, LOU Jian-shi

2009, 14(2):  144-149. 

Asbtract ( 64 )   PDF (284KB) ( 148 )  

References | Related Articles | Metrics

AIM: To study the effects of noninvasive delayed limb ischemic preconditioning (NDLIP) in protecting cerebral tissue from ischemia reperfusion injury.METHODS: NDLIP were performed in rats by three cycle of 5 min ischemia reperfusion of left hind limbs once a day for three days.Animals were divided into four groups :sham group, ischemia reperfusion (I R) group, early cerebral ischemic preconditioning +I R (ECIP +I R) group and NDLIP +I R group.The effects of ischemia reperfusion neurologic defect symptom, cerebral infarction size, superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and xanthine oxidase (XOD), content of malonaldehyde (MDA) in cerebral tissue were observed.RESULTS: Compared with I R group, the neurological score and cerebral infarction size in ECIP +I R group and NDLIP +I R group were markedly lower than those in I R group after ischemic 1 h reperfusion 24 h.Compared with I R group, the activities of T-SOD, Mn-SOD and GSH-PX in ECIP +I R group and NDLIP +I R group increased, while the XOD activity and MDA content obviously decreased, and there were no significant difference between ECIP +I R group and NDLIP +I R group.CONCLUSION: NDLIP could decrease the nerve injury and cerebral infarction size, increase the ability of anti oxidation after I R, and protect cerebral tissue injury of I R.

Effects of iptakalim, a novel ATP-sensitive potassium channel opener, on the phosphorylation of ERK1/2 in primary cultured human pulmonary arterial smooth muscle cells

MEI Hong-bo, XIE Wei-ping, ZUO Xiang-rong, WANG Hong

2009, 14(2):  150-154. 

Asbtract ( 58 )   PDF (271KB) ( 84 )  

References | Related Articles | Metrics

AIM: To study the effects of iptakalim, a novel ATP-sensitive potassium channel (KATP) opener, on the phosphorylation of extracellular signalregulated kinase1/2 (ERK1/2) induced by endothelin- 1(ET-1) in primary cultured human pulmonary arterial smooth muscle cells.METHODS: By Western blot analysis, the phosphorylation level of ERK1/2 was measured in primary cultured human pulmonary arterial smooth muscle cells.The cells were treated with ET-1 (10 nmol/L) for 0, 1, 2, 5, 10, 30, 60 min, respectively.The cells were pretreated with 0.1, 1.0 and 10 μmol/L iptakalim respectively for 30 min prior to the treatment with ET-1 (10 nmol/L) for 10 min.The cells were pretreated with Glibenclamide(10 μmol/L) for 30 min prior to the treatment with ET-1 (10 nmol/L) and iptakalim (10 μmol/L) for 10 min.RESULTS: ET-1 induced phosphorylation of ERK1/2 from 2 to 30 min with a peak response observed at 10 min in a time-dependent manner.Iptakalim inhibited ET-1-induced ERK1/2 phosphorylation in a concentration-dependent manner.Glibenclamide, a selective KATP channel antagonist, could antagonize the effects of iptakalim. CONCLUSION: Iptakalim inhibited ET-1-induced phosphorylation of ERK1/2 in primary cultured pulmonary arterial smooth muscle cells probably through activating KATP channel and would be a most promising candidate drug to treat the remodeling of pulmonary vasculature and pulmonary arterial hypertension.

Anti-inflammatory effects of the compatibility of Radix Asteris and Flos Farfarae

LI Cong, HUANG Fang, DOU Chang-gui, ZHANG Mian, MA Shi-ping

2009, 14(2):  155-159. 

Asbtract ( 92 )   PDF (206KB) ( 246 )  

References | Related Articles | Metrics

AIM: To investigate the anti-inflammatory effects of the mixture of the active parts of Radix Asteris (RA) and Flos Farfarae (FF) (1∶1, w/w) and analyze the relation of compatibility between them. METHODS: The anti-inflammatory effects were measured by inhibiting the nitric oxide synthesis in LPS-ac-tivated macrophageshe, inhibitioning of capillary permeability inmice and acute airway inflammation caused by centrated ammonia method in mice.RESULTS: Compared with two individual active parts, the mixture playded a more prominent role in inhibition of capillary permeability in mice (inhibition ratio :45.1 %, 45.3 %, 51.8 %) and acute airway inflammation caused by centrated ammonia method in mice (inhibition ratio :48.0 %, 53.4 %, 61.4 %);furthermore, the same result resided in RA2, FF3 and their mixture in inhibition of nitric oxide synthesis in LPS-activated macrophageshe(P <0.05 or P <0.01).CONCLUSION: The mixture of two active parts of RA and FF can significantly strengthen the anti-inflammatory effect of two individual active parts, indicating that the mixture of two active parts is provided with mutual promotion effect.

Research on combination of the Radix Asteris with Flos Farfarae and the effect of Radix Asteris for expectorant

LI Na, MA Shi-ping, HUANG Fang, ZHANG Mian, DOU Chang-gui

2009, 14(2):  159-162. 

Asbtract ( 75 )   PDF (160KB) ( 265 )  

References | Related Articles | Metrics

AIM: To investigate the effective parts of Radix Aster and synergistic effects of Flos Farfarae by eliminating phlegm experiment.METHODS: Radix Aster, Flos Farfarae extractionmethods were established as 30 % ethanol backstreaming extraction and water extraction, respectively, indexed with phenol red excretion.The extractive was precipitated using alcohol and 3 parts were extracted :60 % ethanol parts, 90 % ethanol parts, and 90 % ethanol solution parts.RESULTS: The effective fraction of Radix Asteris was 90 % ethanol parts (RA2), the synergistic fraction of Flos Farfarae was 90 %ethanol solution parts (FF3). CONCLUSION: The effective fraction of Radix Asteris is in high polar region, the synergistic fraction of Flos Farfarae is in low polar region.

Effects of Suyu capsule on behaviors and hypothalamus-pituitary-adrenal axis in depression model rats induced by bilateral olfactory bulb impairment

HUANG Zhen, MAO Qing-qiu, PAN Ai-juan, FENG Chun-rong, LI Zhao-yi, ZHONG Xiao-ming

2009, 14(2):  163-166. 

Asbtract ( 73 )   PDF (177KB) ( 89 )  

References | Related Articles | Metrics

AIM: To study the effects of Suyu capsule on behaviors and hypothalamus-pituitary-adrenal axis in depression model rats induced by bilateral olfactory bulb impairment.METHODS: 48 male SD rats were randomly divided into 6 groups including the sham group, the model group, and three Suyu capsule groups(The doses were 22.8, 11.4, 5.7 g/kg, respectively) and Clomipramine group(0.02 g/kg).The depression model was established by bilateral olfactory bulb impairment.The behaviors of rats were observed using open-field and step-down test.The levels of CRH, ACTH and CORT in plasma of rats were detected by radioimmunoassay (RIA).RESULTS: Suyu capsule could decrease the numbers of crossing and rearing of depression model rats using the Open-Field test.Suyu capsule increased the latent time and diminish the mistakes for the depression model rats in the step-down test, Suyu capsule also decreased the levels of CRH, ACTH and CORT in plasma of depression model rats.CONCLUSION: Suyu capsule could reverse the behavioral changes of depression model rats induced by bilateral olfactory bulb impairment, which may be related to Suyu capsule effectively antagonize hypothalamus-pituitary-adrenal axis hyperfunction.

Effect of thalidomide combined with methotrexate on the inflammation and cytokines of rats with collagen type II induced arthritis

ZHOU Wei, ZHANG Yu, SHEN Wei-gan, XUE Yong-ji, GAO Na, GAO Bo, ZHANG Xue-zhen

2009, 14(2):  167-170. 

Asbtract ( 66 )   PDF (204KB) ( 86 )  

References | Related Articles | Metrics

AIM: To investigate the effect of thalidomide combined with methotrexate on the inflammation and cytokines of rats with collagen type II induced arthritis.METHODS: The model of rat was induced by injection of collagen type II combined with complete Freund's adjuvant (CFA).Next day after the injection, the therapeutic administration of thalidomide(150 mg·kg^-1 ·d^-1) combined with methotrexate (0.2 mg·kg^-1 ·d^-1) and methotrexate(0.2 mg·kg^-1·d^-1) were differently performed to the rats.The courses of the treatment were all six weeks.The mean arthritis index (AI) was scored every week.The relative concentrations of IL-1β, TNF-αin sera were evaluated by Western Blotting.RESULTS: The therapeutic administration of thalidomide combined with methotrexate and methotrexate could decrease the mean arthritis index on CIA rat, the effect of thalidomide combined with methotrexate was more obvious than that of methotrexate. The administration of thalidomide combined with methotrexate and methotrexate significantly decreased the production of IL-1β and TNF-α, the change of posttreatmentis was obviously in all groups (P <0.05). CONCLUSION: Thalidomide combined with methotrexate have an inhibitory effect on the expression of IL- 1β and TNF-αin model rat, which is related to its anti- inflammation.There are coordinate effect in thalidomide and methotrexate.

Experimental study on effects of component from Centella asiatica on compound TLR4 in mice tubular epithelial cells stimulated by tumor necrosis factor α

ZHU Xiao-ling, WANG Yong-jun, YANG Ru-chun, LIN Yi, YU Dong-rong, LU Ying, LI Ke, ZHOU Wu-ding

2009, 14(2):  171-174. 

Asbtract ( 70 )   PDF (209KB) ( 92 )  

References | Related Articles | Metrics

AIM: To investigate the effects of component from Centella asiatica (centella asiatica & emodin) on toll like receptor 4 (TLR4) mRNA and protein in mice tubular epithelial cells (mTEC) stimulated by tumor necrosis factor α(TNF-α).METHODS: Cultured mTEC were exposed to TNF-α5 ng/mL and centella asiatica &emodin for 24 hours, followed by transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked flow cytometry (FCM) analysis for complement TLR4 mRNA and protein expression.RESULTS: TLR4mRNA and protein production were present in mTEC.TNF-αup-regulated TLR4 production in mTEC.Centella asiatica combined with emodin synergistically down-regulate TLR4 expression at a dose of 4 μg/mL and 0.4 μg/mL.CONCLUSION: Effective component from Centella asiatica component induced TLR4 stimulated by TNF-αin source of kidney local.

Clinical study on efficacy and safety of mitiglinide on type 2 diabetes mellitus

LV Xiao-feng, YAO Lu, ZHU Lv-yun, LIU Jun-jiang, CHAI Jian-guo, ZGANG Shan-fei

2009, 14(2):  175-179. 

Asbtract ( 81 )   PDF (201KB) ( 218 )  

References | Related Articles | Metrics

AIM: To evaluate the efficacy and safety of domestic mitiglinide and imported nateglinide. METHODS: The study was designed in double blind and double simulation fashion.218 patients of type 2 DM were randomly treated with either mitiglinide or nateglinide.The treatment course was 16 weeks.Before the treatment and after 16 weeks of the treatment, the blood glucose of 2 hours after meal, HbA1C, fasting plasma glucose in vein and FBG were detected as the efficient index.The adverse event, adverse reaction incident, blood pressure, heart rate, blood and urine routine, function of Liver and Kindey, ECG were detected as the safety index.RESULTS: Blood glucose of 2 hours after meal, HbA1C, fasting plasma glucose in vein and FBG were decreased in both groups after treatment, efficacy, adverse event, adverse reaction incident, blood press, heart rate, blood and urine routine, function of Liver and Kindey, ECG were no distinct difference between mitiglinide and nateglinide P 0.05.CONCLUSION: The curative effect of domestic mitiglinide is exact, efficacy and the tolerance is favourable.

Effects of rosiglitazone on the synthesis of tumor necrosis factor-alpha and tissue factor in peripheral blood monouclear cells from patients with acute coronary syndrome without diabetes

WU Xu-bin, QIAN Zong-jie, WU Guang-wei, LIN Ying-zhong, HU Chang-xin

2009, 14(2):  180-185. 

Asbtract ( 78 )   PDF (263KB) ( 81 )  

References | Related Articles | Metrics

AIM: To study the effects of rosiglitazone on the synthesis of tumor necrosis factor-alpha (TNF-α), tissue factor (TF), and tissue factor activity (TFA) in peripheral bloodmonouclear cells(PBMC) from patients with acute coronary syndrome (ACS) without diabetes.METHODS: The PBMC from patients with ACS without diabetes were isolated and incubated with rosiglitazone (0.0, 0.1, 1.0, 10.0 μmol/L, respectively) for 24 hours.The concentrations of TNF-αin culture medium and the levels of mononuclear cell tissue factor (MCTF) were measured by using enzyme-linked immunosorbent assay (ELISA), and the mRNA expressions in monocytes were detected by reverse- transcriptase polymerase chain reaction (RTPCR), and TF activity was determined by chromogenic substrate assay.RESULTS: Rosiglitazone(0.0, 0.1, 1.0, 10.0 μmol/L) dose-dependently suppressed the synthesis of TNF-α, TF, and TFA in PBMC from patients with ACS without diabetes.In correspondence with the concentrations of 0.0 、0.1 、1.0 、10.0 μmol/L rosiglitazone, the levels of TNF-αprotein were respectively (306 ±40), (262 ±32), (236 ±28), (194 ± 23) ng/L, the ratios of relative semi-quantitative absorption value of TNF-αmRNA expression were respectively (0.78 ±0.14), (0.60 ±0.09), (0.39 ± 0.11), (0.30 ±0.11), the antigens levels of synthesis of TF were respectively (5.8 ±1.3), (4.6 ±0.9), (3.3 ±0.5), (3.0 ±0.3) ng/L, the ratios of relative semi-quantitative absorption value of TF mRNA expression were respectively (0.42 ± 0.11), (0.37 ± 0.10), (0.31 ±0.09), (0.22 ±0.08) and the expressions of TFA were (16.2 ±3.2), (8.6 ±2.0), (5.4 ±0.6), (4.4 ±0.8) pmol/L (all P <0.05). CONCLUSION: Our findings show that rosiglitazone reduces the synthesis of TNF-α, TF, and TFA in PBMC from patients with ACS without diabetes, that indicates rosiglitazone has the antiinflammatory and antithrombotic effects on ACS besides reducing blood glucose, and it might play a potential and important role in prevention and therapy in ACS.

Therapeutic effects of mycophenolate mofetil in refractory nephrotic syndrome

HUANG Yun-jian, WANG Zi-hua, ZHANG Jing-bo, CHEN Feng, ZHAO Jing-hong

2009, 14(2):  186-190. 

Asbtract ( 56 )   PDF (199KB) ( 100 )  

References | Related Articles | Metrics

AIM: To approach the therapeutic effects of mycophenolate mofetil (MMF) in hormonal resistance nephrotic syndromes.METHODS: Patients were diagnosed as nephrotic syndrome and treated with prednisone at the dose of 1 mg·kg ^-1·d ^-1 for over 8 weeks, and 24 patients with unsatisfactory results or were palindromic were selected, and several patients in the 24 patients had been treated with cyclophosphamide or cyclosporine A.All patients were treated with MMF combined with low dose hormone.The initial dose of MMF was 1.0 -1.5 g/d for 3 months, later the dose were reduced, and the maintenance dose of MMF was 0.5 -1.0 g/d, the dose of prednisone was 5 -20 mg/d, the follow-up visit period more than six months. The changes on urine protein, serum albumin, liver function, renal function were compared before and after treatment.RESULTS: Before and after treatment, urine protein decreased from(3.4 ±1.7) g/d to(0.9 ± 0.2) g/d, serum albumin increased from(19.6 ±5.4) g/L to (36.1 ±7.7) g/L.serum creatinine level decreased from(105.7 ±6.4) μmol/L to (90.1 ±5.8) g/L.20 patients(83.3 %) pathogenetic condition were relieved, 15 patients(65.2 %) were with complete remission.5 patients (20.8 %) were partially recovered, and 4 patients (16.6 %) had no response.The adverse effects were observed, including gastrointestinal events (n =8, 33.3 %), bacterial pneumonia (n =4, 16.6 %), herpes zoster (n =1, 4.1 %), hepatic function mild damage (n =3, 12.5 %).CONCLUSION: It is safe and effective to combine MMF with low dose hormone in treatment of hormonal resistance nephrotic syndrome, which could become a therapeutic option for refractory nephrotic syndrome(RNS).

Reduced glutathione hormone's impact on alcoholic hepatitis patient's index of hepatic fibrosis

ZHOU Hong-ping, ZHUANG Rang-xiao, RU Ren-ping

2009, 14(2):  191-194. 

Asbtract ( 92 )   PDF (167KB) ( 237 )  

References | Related Articles | Metrics

AIM: To observe the changes of hepatic fibrosis index, clinical symptom, physical sign and hepatic function after the patients were treated treatment with reduced glutathione hormone.METHODS: The 72 patients were divided into two groups that one was the treatment group with 37 patients and the other was the control group with 35 patients.The control group was accepted the treatment with Ganlixin Injection, Magnesium Aminosuccinate Injection and Mulvital, etc.The treatment group was accepted reduced glutathione hormone(1.5 g, once a day) in addition. The course of treatment lasted for 8 weeks.We observed the two groups'blood-serum fibrosis indexes (HA, LN, PIII-P, C-IV) and biochemistry indxes (TBIL, ALT, AST, GGT) before and after the treatment.RESULTS: The improvements of the treatment group were much better than the control group on the sides of clinical symptom, physical sign, hepatic function and blood-serum fibrosis.The treatment group's blood-serum fibrosis indexes were much lower after the treatment(P <0.01), and there was no clear decline in the control group.There were obvious differences between the two groups(P <0.01).CONCLUSION: Reduced glutathione hormone has the significant effects of anti-fibrosis and liver protection.

Clinical features and prognosis of obstructive and non-obstructive hypertrophic cardiomyopathy patients

TANG Yuan-yuan, TANG Bo-jian, YAN Xiao-wei, FANG Quan

2009, 14(2):  195-198. 

Asbtract ( 80 )   PDF (174KB) ( 271 )  

References | Related Articles | Metrics

AIM: To compare and analyse the clinical features and outcomes between obstructive and non-obstructive hypertrophic cardiomyopathy (HCM). METHODS: We made a retrospective analysis of the clinical data of 112 patients(54 patients were obstructive HCM and 58 patients were non-obstructive HCM)) admitted in our hospital from 2003 to 2006 with the diagnosis of HCM, and followed up for (4.5 ±2.8) years.RESULTS: There were no significant differences in age, family history and complications between obstructive and non-obstructive HCM patients.The proportion of female patients was markedly higher in the obstructive HCM patients than that in the non-obstructive HCM patients.Besides chest distress and heart murmur were more obvious in the obstructive HCM patients, there was no significant difference between the two groups as to other clinical symptoms, signs and clinical outcome.The differences in systolic anterior motion of the mitral valve(SAM sign), maximum pressure gradient in the left ventricular outflow tract (LVOT), left ventricular ejection fraction(LVEF), left atrial diameter, mitral valve regurgitation reached sinificance between the obstructive HCM group and the non-obstructive HCM group.The incidence of clinical events was low in follow-up period, and there was no difference between the two groups.CONCLUSION: There were no significant differences in age, family history and, complications between obstructive and non-obstructive HCM patients.After β blocker-based drug treatment, the incidence of clinical and cardiac events were relatively low.

Effects of inhalation of budesonide suspension and compound ipratropium bromide solution with oxygen driven nebulizers on patients with acute exacerbation of chronic obstructive pulmonary disease and type II respiratory failure

HE Jian-bo, YAN Sun-shun, XU Hui, DAI Yuan-rong, WU Cheng-yun, WENG Hai-xia, WU Li-qin

2009, 14(2):  199-202. 

Asbtract ( 55 )   PDF (179KB) ( 119 )  

References | Related Articles | Metrics

AIM: To study the effect of inhalation of budesonide suspension and compound ipratropium bromide solution with oxygen driven nebulizers on arterial blood gas analysis and lung function of patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and respiratory failure type II and to observe the side effects.METHODS: Thirty AECOPD hospitalization patients were selected in the study, each one inhaled 2 mL budesonide suspension and 2.5 mL compound ipratropium bromide solution with oxygen driven nebulizers (oxygen flow 5 L min) for about 20minutes.Arterial blood gas and peak expiratory flow (PEF) were measured before inhalation of budesonide suspension and compound ipratropium bromide solution and half an hour after inhalation finished, at the same time, the percutaneous oxygen saturation, consciousness, breathing, heart rate were monitored.RESULTS: The inhalation of budesonide suspension and compound ipratropium bromide solution led to the increase in PaO² and PEF, a decrease in PaCO². There were significantly positive correlations between the improvement rate of PEF and the decrease of PaCO².The side effects were tolerable.CONCLUSION: Inhalation of budesonide suspension and compound ipratropium bromide solution with oxygen driven nebulizers could treat the patients with AECOPD and respiratory failure type II with hypoxia and hypercapnia by improving their lung function.The usage of oxygen driven nebulizers has good efficacy and safety for the patients with AECOPD and respiratory failure type II.

Variations of pregnancy-associated plasma protein-A and C-reactive protein in essential hypertension patients with left ventricular hypertrophy

FAN Jian-feng, TANG Sheng-xing, WAN Jun, ZHU Wei-ping, LI Bi-long, ZHU Zheng-cai

2009, 14(2):  203-206. 

Asbtract ( 66 )   PDF (173KB) ( 95 )  

References | Related Articles | Metrics

AIM: To investigate the variation of pregnancy-associated plasma protein-A (PAPP-A) and C-reactive protein (CRP) in essential hypertension (EH) patients with left ventricular hypertrophy(LVH). METHODS: 49 patients with EH were examined by echocardiography to measure the inter-ventricular septum thickness (IVST), left ventricular posterior wall thickness(LVPWT) and left ventricular diastolic diameter(LVDD), and to calculate the left ventricular mass index (LVMI).The patients were divided into the left ventricular hypertrophy (LVH) group and the non-LVH group according to the diagnostic criteria of left ventricular hypertrophy.The levels of PPAP-A were detected by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) and the levels of hs-CRP were performed by double antibody radioimmunoassay. RESULTS: The levels of PAPP-A and hs-CRP were higher in the LVH group than the non-LVH [ 8.28 (5.90 ～ 30.91) mIU/L vs 4.39 (3.02 ～ 6.98) mIU/L, 8.58(0.90 ～ 35.8) mg/L vs 1.90 (0.50 ～ 2.23) mg/L, P all <0.05].The relevant analysis showed that PAPP-A and LVMI were significantly correlated (r =0.649, P <0.05);hs-CRP and LVMI were significantly correlated (r =0.441, P <0.05). CONCLUSION: The levels of PAPP-A and hs-CRP were higher in the LVH group than those in the non- LVH group.PAPP-A and LVMI are positively correlated, hs-CRP and LVMI are also positively correlated. PAPP-A and CRP may be involved in the formation of LVH in patients with EH.PAPP-A and CRP may be as predictors of the EH patients with LVH

A control study of duloxetine and sertraline in the treatment of patients with depression

SHEN Xin-hua, QIAN Min-cai, LIN Min, FEI Xiao-cong, WAN Shi-liang

2009, 14(2):  207-209. 

Asbtract ( 131 )   PDF (140KB) ( 293 )  

References | Related Articles | Metrics

AIM: To study the efficacy and safety of duloxetine in the treatment of patients with depression.METHODS: In this 6-week randomized control trial, patients meeting CCMD-3 criteria for depressive episode were randomizedly divided to duloxetine group (60 mg/d, n =48) and sertraline group (50 -150 mg/d, n =50).Clinic effectiveness was evaluated using HAMD, adverse reactions and safety were assessed with TESS and laboratory examination.RESULTS: The remission rate and response rate were 39.6 % and 68.8 % for duloxetine group and 30.0 % and 58.0 % for sertraline group respectively, without statistically significant difference between two groups.But duloxetine- treated patients showed significantly greater improvement in the HAMD total score at week 1, 2, 4 respectively.The main adverse reactions were lack of appetite, dry mouth, sweating, constipation, nausea and vomit.The dropped out rates were 6.3 % for duloxetine group and 8.0 % for sertraline group respectively, without statistically significant difference between two groups.CONCLUSION: Duloxetine appeared to be a safe and effective treatment for depression.

Hand-foot syndrome induced by chemotherapy drugs

ZHANG Shui-hua, MEI Qi-bing, PAN Xue-ying, MA Jing

2009, 14(2):  210-213. 

Asbtract ( 82 )   PDF (154KB) ( 137 )  

References | Related Articles | Metrics

Hand-foot syndrome is a cutaneous toxic reaction that is often induced by chemotherapy drugs, many chemotherapy drugs have been reported to cause the condition to the different degree.This review introduces clinical manifestation, toxic classification, frequent chemotherapy drugs, pathogenesis, preventive and treatment measures for HFS.

Research status quo and development of herbs and chemical medicines interactions

LIU Zhi-feng, ZHOU Hong-hao

2009, 14(2):  214-219. 

Asbtract ( 62 )   PDF (212KB) ( 109 )  

References | Related Articles | Metrics

Herbs are naturally occurring drugs which were extracted from plants, generally, they come from traditional Chinese medicine.Recently, there are a drastic growth in the combination use of herbs and chemical medicines, which leads to a large number of drug interactions and adverse effects.The CYP450 enzymes and some ABC transporters in human body play an important role in these interactions.Further and deeper researches in this field would be necessary and important to decrease such interactions and guide the safe and rational use in clinic.The paper introduces the drug interactions and latest study progress of common herbs and chemical medicines, it will offer a reference for the research.

Study progress on dose problem and pharmacokinetics of commonly used special antidote

SONG Ya-juan, LIU Yang, DING Guo-hua

2009, 14(2):  220-224. 

Asbtract ( 68 )   PDF (185KB) ( 153 )  

References | Related Articles | Metrics

To investigate the problems about dosage and pharmacokinetics of special antidotes in applications.Collected and analyzed the references about clinical applications, dosage correlative adverse reactions, pharmacokinetics and assaying methods of special antidotes in the last 30 years.Though special antidotes have significant effects in the clinical treatment of poisoning, theirs secondary damages and adverse reactions caused by inadequacy dose or overdose are also more prominent.The problems about the dosage of special antidotes in the clinical application are still the key to successful deintoxication.To handle the dosage accurately in clinical use, and to study their pharmacokinetics is necessary.

Association of aldose reductase and its genetic polymorphisms with diabetic chronic complications

CHENG Huan-lian, OUYANG Dong-sheng

2009, 14(2):  225-229. 

Asbtract ( 57 )   PDF (182KB) ( 93 )  

References | Related Articles | Metrics

Diabetic chronic complications (DCC) are severely harmful to the health of human, and are major causes leading to death and deformity from diabetes.Aldose reductase (AR), a rate limiting enzyme in polyol metabolic pathway, has implicated in the pathogenesis and development of DCC.The polymorphisms in AR gene are proved to be ethnic different.These polymorphisms are related to DCC susceptibility.The investigation on the polymorphisms may be helpful to prevention and therapy of DCC.

Research progress of thalidomide in combination therapy for the treatment of tumor

YU Shan, JI Hui

2009, 14(2):  230-236. 

Asbtract ( 89 )   PDF (238KB) ( 258 )  

References | Related Articles | Metrics

Following the reports of its teratogenicity, thalidomide was banned from the market in the 1960s.Later, researches discovered the teratogenicity was caused by inhibiting angiogenesis, and at that time the antiangiogenesis effect was considered an important factor in anti-tumour.Since then, many other anti-tumour mechanisms of thalidomide had been revealed and thalidomide was considered a kind of potential antitumor drug and was researched widely.This article focuses on the anti-tumour mechanisms of thalidomide and its use in combination therapy for the treatment of solid tumor.

Effects of sodium-calcium exchanger and its inhibitors on cerebral ischemia

ZENG Wen-jun, SHEN Lei, WU Yu-lin

2009, 14(2):  237-240. 

Asbtract ( 97 )   PDF (166KB) ( 98 )  

References | Related Articles | Metrics

In neural cell, Na⁺/Ca²⁺ exchanger (NCX)is thought to play an important role in the regulation of intracellular Ca²⁺ concentration.During the cerebral ischemia/reperfusion, the influxion of Ca²⁺ though NCX reverse mode induce calcium overload, which make neural cell damage.Recently, the benzyloxyphenyl derivatives have been developed as selective NCX inhibitors, which may have therapeutic potential as a new remedy for cerebral ischemia.